A brief overview of the Bigelow Laboratory facilities and their research

Price, Nichole N.

English

University of Maine

The University of MaineDigitalCommons@UMaineAnnual Maine Aquaculture R&D and EducationSummits Conferences and Summits1-14-20152015 AQ Summit: Research Update by NicholePriceNichole N. PriceFollow this and additional works at: https://digitalcommons.library.umaine.edu/ari_rd-edPart of the Aquaculture and Fisheries CommonsThis Presentation is brought to you for free and open access by DigitalCommons@UMaine. It has been accepted for inclusion in Annual MaineAquaculture R&D and Education Summits by an authorized administrator of DigitalCommons@UMaine. For more information, please contactum.library.technical.services@maine.edu.Repository CitationPrice, Nichole N., "2015 AQ Summit: Research Update by Nichole Price" (2015). Annual Maine Aquaculture R&D and EducationSummits. 13.https://digitalcommons.library.umaine.edu/ari_rd-ed/13Sharing	  Culture:	  	  What	  can	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  do	  for	  you?	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Nichole	  N.	  Price	  Collaborators:	  	  Bigelow	  Services	  -­‐	  Steve	  Archer,	  Director	  of	  Bigelow	  Analy>cal	  Services	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  	  Tim	  Pinkham,	  	  Seawater	  Facili>es	  Technician	  	  Mike	  Lomas,	  Director	  of	  Bigelow	  Na>onal	  Center	  for	  	  	   	   	  Marine	  Algae	  and	  Microbiota	  	  Nicole	  Poulton,	  Director	  of	  the	  Facility	  for	  Aqua>c	  	  	   	   	  Cytometry	  Senior	  Research	  Scien=st	  Ocean	  Health	  and	  Ecosystem	  Func>on	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Outline:	  4	  Bigelow	  Laboratory	  	  Core	  Facili>es:	  	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  	  Bigelow	  Analy>cal	  Services	  (BAS)	  	  Bigelow	  Seawater	  Facility	  	  Facility	  for	  Aqua>c	  Cytometry	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Outline:	  4	  Bigelow	  Laboratory	  	  Core	  Facili>es:	  	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  	  Bigelow	  Analy>cal	  Services	  (BAS)	  	  Bigelow	  Seawater	  Facility	  	  Facility	  for	  Aqua>c	  Cytometry	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health NCMA:	  the	  largest	  and	  most	  diverse	  collec=on	  of	  microalgae	  in	  the	  world.	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health ~3000	  Microalgal	  strains	  from	  every	  ocean.	  Maintained	  by	  3	  curators,	  Julie	  Sexton,	  Tracey	  Riggens,	  Jeﬀ	  Brown,	  who	  have	  >50	  yrs	  experience	  culturing	  algae	  collec>vely.	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health NCMA	  also	  has	  159	  macroalgal	  strains	  5	  classes:	  	  Bangiophyceae	  	  Florideophyceae 	  	  	  Rhodophyceae	  	  Ulvophyceae	  	  Phaeophyceae	  >30	  genera:	  	  Palmaria	  decipiens	  	  Porphyra	  plocamiestris	  	  Saccharina	  la3ssima	  	  Laminaria	  digitata	  	  Products	  and	  Services	  	  •  Strains	  provided	  as	  starter	  cultures	  •  Clean	  seawater	  and	  a	  wide	  range	  of	  growth	  media	  kits	  •  Culturing	  Techniques	  Courses	  and	  consul>ng	  on	  growth	  op>miza>on	  •  New	  isola>ons/strain	  clean	  up/taxonomic	  ID	  •  Cryopreserva>on	  and	  perpetual	  culture	  maintenance	  •  Private	  collec>ons	  and	  Interna>onal	  Depository	  Authority	  (patent	  depository)	  •  Research	  services	  (e.g.,	  Nucleic	  acids	  from	  algae,	  mass	  culture,	  macroalgal	  culture,	  growth/compound	  op>miza>on,	  nutri>onal	  analyses)	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health REGISTRATION	  NOW	  OPEN	  !!!	  hPps://ncma.bigelow.org/training-­‐courses	  2015	  Algal	  Culturing	  Techniques	  Course	  Oﬀered	  May	  31-­‐June	  5	  	  Bigelow	  Laboratory	  Research	  and	  Educa>on	  campus	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Outline:	  4	  Bigelow	  Laboratory	  	  Core	  Facili>es:	  	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  	  Bigelow	  Analy>cal	  Services	  (BAS)	  	  Bigelow	  Seawater	  Facility	  	  Facility	  for	  Aqua>c	  Cytometry	  Bigelow	  Analy=cal	  Services	  •  Fee-­‐for-­‐service	  analy>cal	  facility	  •  Serving	  research,	  industry	  and	  State	  •  Open	  to	  new	  ideas	  and	  challenges	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Analy=cal	  capabili=es:	  •  Colorimetric	  analysis	  •  UV/Vis	  spectrometry	  and	  ﬂuorometry	  •  Elemental	  analyzer	  Elementary	  •  Liquid	  chromatography	  (HPLC)	  •  Gas	  chromatograph	  -­‐	  mass	  spectrometry	  •  Liquid-­‐	  chromatography	  -­‐	  mass	  spectrometry	  Compound	  composi>on	  •  Radioisotope	  approaches	  	  •  Stable	  isotope	  approaches	  (compounds	  and	  bulk	  isotope	  ra>os)	  Produc>on	  Rates	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Example:	  Biotoxin	  Tes=ng	  	  0	  10	  20	  30	  40	  50	  60	  70	  0	   5	   10	   15	   20	   25	  LU	  Minutes	  GTX-­‐4	  	  GTX-­‐1	  	  GTX-­‐3	  	  GTX-­‐2	  	  dcGTX-­‐3	  	  dcGTX-­‐2	  	  NEO	  	   dcSTX	  	   STX	  	  Comparison	  of	  primary	  standards	  and	  mussel	  matrix-­‐matched	  standards	  of	  saxitoxins,	  gonyautoxins	  and	  neosaxitoxins.	  	  Microalgal	  toxins:	  Paraly>c	  and	  Amnesic	  Shellﬁsh	  Toxins	  (PSPs	  and	  ASPs)	  Approach:	  •  HPLC-­‐PCOX	  method	  	  •  Maine	  1st	  in	  US	  to	  adopt	  chemical	  analysis	  Conduc>ng	  PSP	  and	  ASP	  analysis	  for	  MEDMR	  shellﬁsh	  monitoring	  program	  (2013-­‐2014	  season).	  Funding:	  Sewall	  and	  Ingalls	  Founda>ons	  	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Example:	  Nutri=onal	  analyses	  e.g.	  Ω-­‐3	  and	  Ω-­‐6	  PUFAs	  	  key	  components	  for:	  •  Growth/development	  •  health	  •  taste	  EPA	  DHA	  Current	  project:	  	  	  Gordon	  and	  Begy	  Moore	  Founda>on	  Polyunsaturated	  Fagy	  Acids	  (PUFAs)	  •  elemental	  analysis	  •  an>oxidants	  •  vitamins	  •  pigments	  •  toxins	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Outline:	  4	  Bigelow	  Laboratory	  	  Core	  Facili>es:	  	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  	  Bigelow	  Analy>cal	  Services	  (BAS)	  	  Bigelow	  Seawater	  Facility	  	  Facility	  for	  Aqua>c	  Cytometry	  Bigelow	  Seawater	  Facility	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Seawater	  system	  Descrip=on:	  •  Located	  on	  the	  Damariscoga	  River	  Estuary,	  Shore	  facility	  and	  ﬂoa>ng	  dock	  w/	  overboard	  pumps	  •  Raw	  and	  Filtered	  Seawater	  Available	  	  –  Pasteurized	  upon	  request	  •  Par>ally	  powered	  by	  20	  kW	  solar	  system	  •  Able	  to	  test	  Ar>c	  to	  Equatorial	  Species	  (can	  control	  light	  and	  temperature)	  •  Systems	  can	  be	  set	  as	  ﬂow-­‐through	  or	  batch	  mode	  	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Seawater	  system	  capabili=es:	  Type	   Capacity	  (L)	   Low	  Temp	  C°	   High	  Temp	  C°	   Temp	  Varia=on	  C°	   #	  of	  Units	  Mesocosm	   2460	   4	   37	   1.5	   6	  Water	  Table	   300	   7.5	   30	   1.5	   3	  Kalwall	  tubes	   94.5	   7	   25	   1	   8	  Bag	  Vessels	   400	   7	   25	   1	   6	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health •  High	  frequency	  monitoring	  of	  seawater	  pH,	  dissolved	  oxygen,	  and	  temperature	  •  Experimental	  manipula>on	  of	  temperature,	  light,	  and	  pH	  (pCO2)	  	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Outline:	  4	  Bigelow	  Laboratory	  	  Core	  Facili>es:	  	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  	  Bigelow	  Analy>cal	  Services	  (BAS)	  	  Bigelow	  Seawater	  Facility	  	  Facility	  for	  Aqua>c	  Cytometry	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health What	  is	  the	  Maine	  algal	  industry	  missing?	  Wordle©	  input	  –	  survey	  summary	  conducted	  by	  Chris	  Davis,	  Maine	  Aquaculture	  Innova>on	  Center	  J.J.	  MacIsaac	  Facility	  for	  Aqua=c	  Cytometry	  100 101 102 103 104Side Scatter (Relative Size)100101102103104Red Relative Fluorescence (Chlorophyll)Sor>ng	  spores	  	  prior	  to	  the	  	  forma>on	  of	  	  gametophytes	  Saccharina	  Life	  Cycle	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Spores	  released	  	  from	  Sporophyte	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Flow	  Cytometry	  and	  Macro-­‐algal	  culture	  Ocean	  Approved	  Kelp	  Manual	  •  Spore	  sor>ng	  and	  gametophyte	  cloning	  •  Cryopreserva>on	  of	  favored	  strains	  	  •  Seeding	  lines	  for	  aquaculture	  •  Sporophyte	  grow-­‐out	  in	  op>mal	  condi>ons	  	  Kawai	  et	  al.	  Nature	  2013	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Michael	  W.	  Lomas	  Director	  Provasoli-­‐Guillard	  Na>onal	  Center	  for	  Marine	  Algae	  and	  Microbiota	  (NCMA)	  Steve	  Archer	  Director	  Bigelow	  Analy>cal	  Services	  Meredith	  White	  Postdoctoral	  Researcher	  	  	  'The	  biological	  responses	  of	  mul>ple	  Northeast	  taxa	  to	  ocean	  acidiﬁca>on’	  3:30	  Friday,	  'Ocean	  Acidiﬁca>on	  II'	  Session	  in	  the	  New	  Hampshire	  Rm	  Visit	  the	  Bigelow	  Booth	  in	  the	  Casco	  Bay	  Exhibit	  Hall!	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Thank	  you	  for	  your	  aPen=on.	  hPps://ncma.bigelow.org	  hPps://www.	  bigelow.org/bas	  hPps://www.bigelow.org/seawater	  hPps://www.bigelow.org/ﬂow-­‐cytometry	  VISION	  	  T o	   c r e a t e	   a n	   o p e r a > o n a l	  environment	   through	   academic,	  p r i v a t e 	   a n d 	   e d u c a > o n a l	  collabora>on	   that	   encourages	  innova>on	   and	   fosters	   the	   vibrant	  growth	  of	  a	  sustainable,	  ecologically	  sound,	   and	   proﬁtable	   macroalgae	  and	   microalgae	   industry	   sector	   in	  Maine.	  Proposal	  for	  a	  Maine	  Algal	  Cluster	  (MAC)	  Ini=a=ve	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health Credit:	  Maine	  Algal	  Cluster	  Ini>a>ve	  proposal	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health What	  are	  the	  perceived	  boPlenecks	  for	  a	  more	  vibrant	  Maine	  algae	  industry?	  Credit:	  Maine	  Algal	  Cluster	  Ini>a>ve	  Steering	  Commigee	  Bigelow	  Laboratory	  for	  Ocean	  Sciences	  Ocean Life, Planet Health 1)  Solve	  the	  discon>nui>es	  	  of	  a	  combined	  macro	  and	  micro	  Algal	  Cluster	  2)  Determine,	  and	  implement,	  the	  Cluster	  organiza>on,	  governance,	  and	  administra>on	  3)  Iden>fy	  further	  R	  &	  D	  needs	  that	  >ghten	  the	  eﬃciencies	  of	  producers/businesses,	  create	  new	  products	  and	  processes	  4)  Iden>fy	  proﬁtable	  markets	  5)  Plan	  for	  produc>on	  scaled	  to	  market	  demand	  6)  Structure	  investment	  to	  fuel	  expansion	  7)  Select	  and	  promote	  educa>on	  and	  outreach	  methods	  in	  support	  of	  commercial	  produc>on	  	  	  	  What	  are	  the	  objec=ves	  of	  the	  Maine	  Algal	  Cluster	  Ini=a=ve?	  

2015 AQ Summit: Research Update by Nichole Price

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2015 AQ Summit: Research Update by Nichole Price

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