The association of eighteen species of Cleridae (Coleoptera) with tallgrass and shortgrass prairie ecosystems in central North America is reported for the first time. New flower visitation, habitat association, distribution, and/or rearing records are reported for Enoclerus analis (LeConte), E. coccineus coccineus (Schenkling), E. cordifer (LeConte), E. rosmarus (Say), E. zonatus (Klug), Placopterus thoracicus pallipes (Wolcott), Trichodes bibalteatus LeConte, T. bicinctus Green, T. nutalli (Kirby), Phyllobaenus dubius (Wolcott), P. knausii (Wickham), P. pubescens (LeConte), P. subfasciatus (LeConte), Isohydnocera albocincta (Horn), I. brunnea (Horn), I. curtipennis (Newman), I. tricondylae (LeConte) and Wolcottia pedalis (LeConte). Diagnostic characters are presented to separate adults of P. dubius, a species endemic to the northern shortgrass prairie region, from the common and widespread P. pubescens
