Arthur, Rodrigo Alex

Degrazia, Felipe Weidenbach

Leitune, Vicente Castelo Branco

Tokimi, A.

Lume 5.8

HA008 Effects of photobiomodulation on the masseter of children withspastic cerebral palsy: a pilot studySantos MTBR*, Pedrique B, Silva KJ, Ortega AOL, Maciel SCUNIVERSIDADE CRUZEIRO DO SUL.E-mail: drsantosmt@yahoo.com.brThis longitudinal study on 20 children with spastic type cerebral palsy (CP), followed by theAssociation for the Care of Disabled Children (Associação de Assistência à Criança Deficiente,AACD), evaluated the effect of photobiomodulation laser on the thickness of their massetermuscles. The Laser Group (LG) comprised 10 children with complaints of mouth openingrestriction and difficulty in performing oral hygiene; the non-Laser Group (nLG) comprised10 children with no restriction. LG was submitted to six infrared laser applications: LED,low intensity, As-Ga-Al, at λ = 808±3 nm, 120 mW, by MMOptics Twin Flex Evolutionlaser; using 10.0 J/cm2 of energy/dose and 20 s of exposure/site, at 7-day intervals. Massetermuscle thickness was evaluated using ACUSON X300 ultrasound equipment (SIEMENS).LG was assessed before any application and after six sessions, while the nLG was assessedtwice, at 7-day intervals. The t tests for dependent and independent samples were used, at a5% significance level. The groups were similar in sex and age, though differences (p=0.003)were verified for the clinical form of CP; LG with tetraparesis showed greater values. Thethickness of the right masseter before (8.9±2.1) and after (9.9±2.9) six laser applicationsdiffered significantly (p=0.039), as did the left (9.1±1.9, 9.7±1.6; p=0.023). Masseter musclemeasurements of nLG and LG after six weeks showed no significant difference (right: p=0.484;left: p=0.395).Photobiomodulation has a positive effect, as verified by the increase in thickness of the spasticmasseter muscles (Apoio: Fundação de Amparo à Pesquisa do Estado de São Paulo - Processo2014/15662‑1)HA009 The osteoprotective effect of estrogen receptor alpha in maxillaryboneMacari S*, Sharma LA, Szawka RE, Dias GJ, Silva TAClínica, Patologia e Cir - UNIVERSIDADE FEDERAL DE MINAS GERAIS.E-mail: soraiamacari@hotmail.comIntroduction: Estrogen deficiency results in systemic bone loss. However, the contributionof estrogen receptor alpha (ERα) in maintenance of alveolar bone microarchitecture andunderlying mechanisms were not defined yet. Methods: 8‑10 weeks old females and maleshomozygote ERα+/+ (wild type - WT) and ERα-/- (ERKOα) mice were submitted tomechanical loading-induced bone remodeling by using an orthodontic appliance to promotetooth movement (OTM). The maxillary bone samples were analysed using microCT, qPCRand energy dispersive spectroscopy (EDS). Bone marrow cells (BMC) from WT and ERKOαmice were isolated and differentiated in osteoblasts and osteoclasts. Results: Both female andmale ERKOα demonstrated an osteoporotic phenotype in the femur and vertebrae. Maxillayalveolar bone loss and OTM were significantly augmented in ERKOα mice and associated withdecreased calcium percentage levels and increased expression of IL-33 in the periodontium. Invitro osteoclasts and osteoblasts differentiation was significantly higher in BMC from ERKOαthan WT mice.In conclusion, we provided herein evidence for the first time that estrogen receptor alpha (ERα)is important to maintain the microarchitecture and control bone remodeling of maxillaryalveolar bone. ERα also protects femur and vertebrae from bone loss, either in female andmale mice. ERα effects might be related to local production of IL-33, but seems independentof RANK/RANKL. ERα has yielded osteoclastogenisis down-regulation which contributes tobone protection. (Apoio: CAPES - PDSE5623/13‑8)HA010 Effect of bioactive primers on dentin-adhesive interfaceSousa ABS*, Bedran‑Russo A, Pires‑de‑Souza FCPMateriais Dentários e Próteses - UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO.E-mail: bia_abss@hotmail.comRecently, it was reported that the degradation of dentin matrix by metalloproteinases (MMPs)could affect the adhesive interface stability. Thus, searching for better results in the longevityof adhesive restorations, the aim of this study was to evaluate the effect of bioactive primers(chlorhexidine - CHX, grape seed extract - GSE, doxycycline - DOXY) on bond strength(BS) of resin composite restorations submitted to load cycling (LC). For this, cavities wereperformed in 48 healthy human molars. Then, it was separated into 4 groups according tosurface treatment received after the acid etching: GI - Control (Adhesive System, AS); GII -CHX 0.2% + AS; GIII - GSE 15% + AS and GIV – DOXY 3% + AS. After, the sampleswere separated into 2 subgroups: A - Control and B - Submitted to LC. Then, the specimenswere submitted to microtensile test andin situ zymography. Assay of collagenase/gelatinaseand gelatin zymography were also performed. According to the statical analysis (2 – wayANOVA, Tukey, p>.05 - SPSS), BS values were not influenced by the use of bioactive primersand by LC (p>.05). In situ zymography analysis demonstrated that the bioactive primersdecreased the activity of MMPs compared to GI (p<.05). GI showed the highest value (p<.05),while no differences was found in the groups treated with bioactive primers (p>.05). Assayof collagenase/gelatinase and gelatin zymography showed that bioactive primers were able toinhibit MMPs, considering CHX less efficient.In conclusion, the bioactive primers could be used in restorative procedures once they did notaffect the BS values and decreased the MMPs activity. (Apoio: CAPES)HA011 A new perspective of bonding agentsNicoloso GF*, Antoniazzi BF, Soares FZM, Rocha ROUNIVERSIDADE FEDERAL DE SANTA MARIA.E-mail: nikoloso_sma@hotmail.comThis study evaluated the bond strength of the adhesive Scotchbond Universal applied either inetch-and-rinse or self-etch strategies compared to Adper Single Bond 2 and Clearfil SE Bond intwo different conditions of dentin (sound and demineralized) commonly observed in minimalinvasive restorations. Forty-eight sound human third molars had mid-coronal dentin surfacesexposed by removing the occlusal third. Twenty-four molars were submitted to pH-cycling tocreate demineralized dentin, and the other half remained intact until the bonding procedure.Eight groups (n=6) were created according to the adhesives tested and conditions of the dentin.After bonding procedures, a composite core was build-up to a height of 4‑5mm and then wassectioned perpendicular to the adhesive interface in order to obtain rectangular sticks (0.8mm2) that were submitted to microtensile tests (1mm/min). Two-way ANOVA and post-hocTukey´s test (α = 0.05) were performed as statistical analysis. Lower bond strength values wereobtained to demineralized dentin (p=0.000), irrespective of the adhesive/strategy evaluated.Scotchbond Universal in both strategies evaluated had similar bond strength values to bothcompared adhesives (p=0.07). A higher number of premature failures (ptf) were observed ingroups that the bonding procedure was performed in demineralized dentin.The new ‘universal’ adhesive can be used in both strategies, irrespective of the substrate,without the concern of jeopardize the restorative procedure to perform minimal invasive resincomposite restorations.HA012 Biomimetic adhesive resin with Triclosan-doped Halloysite nanotubefillersDegrazia FW*, Leitune VCB, Arthur RA, Takimi A, Collares FMLaboratório de Materiais Dentários - UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL.E-mail: fdegrazia@hotmail.comThe aim of this study was to characterize and evaluate the properties of adhesive resinwith Triclosan-doped Halloysite nanotube fillers. Experimental adhesives with Bis-GMA/TEGDMA, 75/25 wt% and CQ/EDAB/DPIHFP 1 mol% as photoinitiators were formulated.Halloysite Nanotubes (HNT), functionalized or not with 1:1 Triclosan (TCN), wereincorporated into the adhesive at 5, 10, and 20 wt%, totalizing 7 groups with one controlgroup without filler addition. Groups were evaluated by Knoop hardness and softening insolvent, surface free energy (SFE) using optical tensiometer, transmission electron microscopy(TEM), polymerization kinetics and degree of conversion (DC) using FTIR spectroscopy,mineral deposition after artificial saliva immersion using Raman spectroscopy (965 cm-1- PO43- peak), and antimicrobial activity against Streptococcus mutans. SFE and Knoophardness increased with higher amount of HNT. TEM showed the presence of TCN particlesin the inner and outer surfaces of HNT. The DC(%) of HNT/TCN 5 (55.37±0.87), 10(57.12±0.06), and 20 wt% (55.97±0.91) increased significantly (p<0.05) comparing to controlgroup (48.50±1.13). Polymerization rate decreased with TCN-doped HNTs. As the HNTcontent increased, mineral deposition increased for all groups after 14 days of immersion.Bacterial growth inhibition was found with HNT/TCN 20 wt%.Addition of HNT with TCN at 20% to adhesive resin was able to promote antimicrobialactivity and mineral deposition without compromising chemical mechanical properties.(Apoio: CAPES)HA013 Novel natural dentin biomodification agent from cashew nut shelland its application as monomer on dentin bondingFeitosa VP*, Souza NO, Lemos MVS, Ogliari AO, Ogliari FA, Maia FJN, Oliveira DLV,Mazzetto SEOdontologia Restauradora - UNIVERSIDADE FEDERAL DO CEARÁ.E-mail: victorpfeitosa@hotmail.comThe aim of this study was to assess the dentin biomodification of proanthocyanidins (PACs,gold standard) and that of cardol (extracted from the cashew nut shell liquid). Demineralizeddentin bars were evaluated for the initial weight and elastic modulus by flexural test. Themodulus was re-evaluated after bars immersion for 60s (n=10) in distilled water (control), 2%PACs or cardol solutions. The samples were then stored in artificial saliva and the weight wasre-evaluated after 1 and 4 weeks. The cardol was reacted with methacrylic acid to synthesizethe new monomer CMA (cardol-methacrylate) which was purified and characterized with gaschromatography. In flat dentin surfaces from extracted molars it was applied phosphoric acidfor 15s, rinsed and it was applied distilled water, solutions of PACs, cardol or CMA (2%each) for 60s prior to the application of Singlebond Universal adhesive. The restored teethwere cut into sticks for the bond strength (µTBS) test. Data were submitted to ANOVA andTukey test (α=95%). The statistical analysis showed that the modulus variation was higherwith cardol (338.2±45.1%) than with PACs (56.3±5%). The weight loss (degradation) with theuse of PACs (38.6±4.3%) was higher than with cardol (21.5±2.9%). The µTBS was increasedfrom 32.3±4 MPa (control) to 41.9±4.3 MPa by using PACs and 44.5±6.5 MPa with cardol.However, the highest µTBS was obtained with CMA (52.3±4.2 MPa).Cardol has demonstrated to be an efficient alternative for dentin biomodification and its analogCMA may be used to significantly improve the bonding to dentin. (Apoio: CAPES)Proceedings of the 32nd SBPqO Annual Meeting Braz Oral Res 2015;29(suppl 1) 33

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Biomimetic adhesive resin with Triclosan-doped Halloysite nanotube fillers

Universidade Federal do Rio Grande do Sul

https://lume.ufrgs.br/bitstream/10183/149921/1/000988364.pdf

Biomimetic adhesive resin with Triclosan-doped Halloysite nanotube fillers

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Lume 5.8

Universidade Federal do Rio Grande do Sul