In this study optimization of extraction conditions for saponin from Sapindus mukorossi was investigated. Results showed that polarity of the extraction solvent affects the yield percentage of the extraction process. Best yield percentage was obtained as 78.1 % at 1:10 solid-liquid ratio in aqueous ethanol solution (50% v/v). The antimicrobial properties of extracts containing saponins were investigated for different microorganisms. Minimum inhibition concentrations of extract were obtained against Escherichia coli, Staphylococcus aureus and Candida albicans. Minimum inhibition concentrations (MIC) of saponin extract ranged between 12.5 mg/mL to 25 mg/mL.nbs