Molecular biological enhancement of coal desulfurization. [Thiobacillus cuprinus]

Abstract

The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original host to enhance the biodesulfurization activity of the organism; transfer this pathway into a fast-growing chemolithotrophic bacterium; conduct a batch-mode optimization/analysis of scale-up variables