Order from chaos: Microfluidic chaotic mixing shows a significant improvement in the equilibration time of hybridization in DNA microarrays over conventional techniques. These results illustrate a new concept for performing kinetics studies in microfluidic devices

Gwirtz, Richele M.

Liu, Jian

Quake, Stephen

Williams, Brian A.

Wold, Barbara J.

Caltech Authors - Main

  
 
 
 
Supporting Information 
© Wiley-VCH 2006 
69451 Weinheim, Germany 
 1
Enhanced Signals and Fast Nucleic Acid Hybridization Using Microfluidic 
Chaotic Mixing** 
Jian Liu, Brian A.Williams, Richele M. Gwirtz, Barbara J. Wold, and Stephen Quake* 
 
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0.0
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t = 2 hr
t = 8 min
t = 4 min
t = 0 min
sample windows along the equator
Fluorescence
     / AU
 
0 10 20 30 40 50 60
0.0
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      / AU
 
2 4 6 8 10
0.0
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t = 2 hr
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Supplementary Fig.5 Evaluation of mixing efficiency of two different devices with 
(a&b)/ without (c&d) the herringbone indentations in the bridge channels. A solution 
containing 0.1m fluorescent beads and a blank solution were loaded into the chambers 
with a pattern similar to Fig.1b. Ten windows along the equator of one chamber were 
specified to monitor the fluorescent intensity changes in real-time. Fig.5a (with 
chaotic mixing) and Fig.5c (without chaotic mixing but with circulation) compare the 
fluorescent changes of these windows in the time course. Fig.5b and Fig.5d show 
a) b) 
c) d) 
 2
different gradient curves at four specified moments (0 min, 4 min, 8 min, 2 hours). 
Chaotic mixing is much more effective at eliminating heterogeneity in the solution 
than simple circulation. The Stokes-Einstein diffusion coefficient of 0.1 m beads was 
estimated to be 4.4x10-8 cm2s-1, within the comparable range of DNA molecules (1k 
bp). 
 


Enhanced Signals and Fast Nucleic Acid Hybridization By Microfluidic Chaotic Mixing

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Enhanced Signals and Fast Nucleic Acid Hybridization By Microfluidic Chaotic Mixing

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