The World Health Organization classified (Williams, 1983) Y. pestis as Y. pseudotuberculosis subsp. pestis on the basis of DNA homology, yet the two organisms cause markedly different disease. Portnoy and Falkow (1981) reported IS100 to influence the virulence of Y. pestis. IS100 was shown to be found in Y. pestis but not Y. pseudotuberculosis.
IS100 from Y. pestis was sequenced and shown by sequence analysis to fulfill the requirements of being an IS element. pIS1C, an 821 bp fragment of IS100 was transferred to Y. pseudotuberculosis Trp-Ca-, resulting in the ability of the cells to ferment rhamnose and delayed production of urease at 37$\sp\circ$C but not at 26$\sp\circ$C. These traits are normally associated with Y. pestis. Maxicell analysis revealed more products and larger products than could be expected from the coding capacity of the clone. Two-dimensional polyacrylamide gel electrophoresis analysis demonstrated a complex rearrangement of protein profile based on the presence of pIS1C. These data clearly indicate the thermo-regulatory involvement of IS100 at the molecular level

Torosian, Stephen Dale

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University of New HampshireUniversity of New Hampshire Scholars' RepositoryDoctoral Dissertations Student ScholarshipSpring 1993Insertion sequence elements in Yersinia:Nucleotide sequence of IS100 of Yersinia pestisStephen Dale TorosianUniversity of New Hampshire, DurhamFollow this and additional works at: https://scholars.unh.edu/dissertationThis Dissertation is brought to you for free and open access by the Student Scholarship at University of New Hampshire Scholars' Repository. It hasbeen accepted for inclusion in Doctoral Dissertations by an authorized administrator of University of New Hampshire Scholars' Repository. For moreinformation, please contact nicole.hentz@unh.edu.Recommended CitationTorosian, Stephen Dale, "Insertion sequence elements in Yersinia: Nucleotide sequence of IS100 of Yersinia pestis" (1993). DoctoralDissertations. 1742.https://scholars.unh.edu/dissertation/1742Reproduced with permission of the copyright owner.  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Insertion sequence elements in Yersinia: Nucleotide sequence of IS100 of Yersinia pestis

https://scholars.unh.edu/cgi/viewcontent.cgi?article=2741&amp;context=dissertation

Insertion sequence elements in Yersinia: Nucleotide sequence of IS100 of Yersinia pestis

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