Do white-tailed deer (Odocoileus virginianus) prefer to eat certain types of acorns based on tannin content?
Hydrolyzable tannins consist of gallate groups connected via ester linkages to a carbohydrate (D-glucose)
The Prussian Blue method was used to determine hydrolyzable tannin conten

Browning, Sarah

Woodruff, Marshall E.

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Extraction and Quantification of Hydrolyzable Tannins in Acorns from Different Species of Oak Trees (Quercus spp.)Sarah E. Browning1, Marshall E. Woodruff2, Russell J. Franks1*1Department of Chemistry & Biochemistry, 2Arthur Temple College of Forestry & AgricultureStephen F. Austin State University, Nacogdoches, TXResults:Hydrolyzable tannin structures: Conclusions:• Results are comparable to those from analysis of acorn samples from Poland3• ATCOFA researchers are testing dietary preferences of white-tailed deer for various acorns in collaboration with the USDAFuture Work:• Determination of condensed tannin content in current acorn samples using the acid-butanol method• Analysis of acorns from other varieties of QuercusAcknowledgements:• SFASU Dept. Of Chemistry & Biochemistry• SFASU Arthur Temple College of Forestry and Agriculture• SFASU Office of Research & Sponsored Programs • Robert A. Welch Foundation(Grant #AN-0008) References:1. Hagerman, A. E. “Modified Prussan Blue Assay for Total Phenols” Tannin Handbook 20022. Price, M.L.; Butler, L.G. J. Agric. Food Chem. 1977, 25, 1268-1273.3. Luczaj, L.; Adamczak, A.; Duda, M. Dendrobiology 2014, 72, 103-111.Introduction:• Do white-tailed deer (Odocoileusvirginianus) prefer to eat certain types of acorns based on tannin content?• Hydrolyzable tannins consist of gallate groups connected via ester linkages to a carbohydrate (D-glucose)• The Prussian Blue method was used to determine hydrolyzable tannin contentMethods:• Acorns were dried and ground into powder• Fats were removed by stirring acorn powder in hexane/heptane• Tannins were extracted overnight at reflux in 80% MeOH/H2O • Extract was analyzed using Prussian Blue method1,2• Spectrophotometric analysis was performed using gallic acid as a standard16S32.0649F18.99833As74.91392U238.030Stephen F. Austin State UniversityDepartment of Chemistry & BiochemistryGallotannin EllagitanninOak Species Total Phenolic Content(mg GAE† / g acorn)Overcup Oak (Q. lyrata) 2.4 ± 0.3Live Oak (Q. virginiana) 3.8 ± 0.8Swamp Chestnut Oak (Q. michauxii) 4.9 ± 0.7White Oak (Q. alba) 5.2 ± 0.5Water Oak (Q. nigra) 5.6 ± 0.7Shumard Oak (Q. shumardii) 6.3 ± 0.8Prussian Blue Reaction:𝟑𝐏𝐏𝐫𝐞𝐝 + 𝟔𝐅𝐞 𝐂𝐍 𝟔𝟑−+ 𝟖𝐅𝐞𝟑+ → 𝟑𝐏𝐏𝐨𝐱 + 𝟐𝐅𝐞𝟒 𝐅𝐞 𝐂𝐍 𝟔 𝟑PPred = reduced form of polyphenolPPox = oxidized form of polyphenolResults of acorn analysis†GAE = gallic acid equivalentCalibration Curve for Prussian Blue Analysis

Extraction and Quantification of Hydrolyzable Tannins in Acorns from Different Species of Oak Trees (Quercus spp.)

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Extraction and Quantification of Hydrolyzable Tannins in Acorns from Different Species of Oak Trees (Quercus spp.)

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