The optimization procedure for the attachment of saccharomyces cerevisiae on the surface of a carbon paste electrode

Abstract

Various kind of organic dyes (Amido black, Phenol red, Rhodamine B, Neutral red, Eriochrome black T, o-cresol, Crystal blue, and fuchsine) were studied on the purpose of indirectly evaluating the attachment process of Saccharomyces cerevisiea on the surface of a carbon paste electrode (CPE). Appropriate conditions for adsorption of the individual organic dyes onto the CPE (concentration, accumulation time, stirring speed) have been optimized. Besides, it has been observed that the current derived from the oxidation of Amido black, which was adsorbed onto the CP-surface after attaching the yeast cells, decreases along with time