Present situation in financing of regional railway transport does not allow sustainable development of transport services in a region and also often leads to further worsening of situation in rolling stock financing. This paper describes basic problems related to financing of regional transport services from
regional budgets. Stability of regional railway transport financing in the future provides the resolution of government of the Czech Republic No. 1132/2009 from the 31st August 2009, for the renewal of rolling stock will be possible to get grants from the Regional operational program (ROP). Regional
authorities have to decide how to prepare the long-term contract concerning the public service obligation. The question is how to choose the operator of regional trains and how to get the highest quality of provided services from amount incurred