Investigating Evolutionary History Using Phylogenomics

Abstract

Reconstructing the Tree of Life is one of the principal aims of evolutionary biology. The development of molecular phylogenetics to elucidate evolutionary history has complemented palaeontology, biogeography, and archaeology in elucidating biological history. The development of molecular-clock analyses allowed evolutionary timescales to be estimated using nucleotide sequences and other products of the evolutionary process Until recently, the twin challenges of molecular dating were in obtaining sufficient data and developing robust methods. The former concern is now less important as high–throughput sequencing technology allows entire genomes to be sampled. Genome–scale data enhances statistical power, but accompanying this wealth of data is a new suite of analytical challenges. One of these key challenges is analysing these data in synthesis with the paleontological record without statistical overparameterisation. There are also aspects of the evolutionary process, such as among–lineage rate variation, that can affect the precision and accuracy of current methods. In this thesis, I first use the richest nucleotide sequence data set of insects available to estimate an authoritative insect evolutionary timescale that dates the origins and diversification of every major insect order. I then focus on molecular-clock methods by testing their performance in inferring evolutionary rates from time–structured data, common in the study of ancient DNA. I find that among–rate lineage variation and phylo–temporal clustering affect rate estimates. I also study data partitioning, a common technique used to optimise the analysis of multilocus data where independent parameters are applied across different subsets of the data. New data from the genomic revolution gifts biologists new opportunities to re-examine enduring questions about the evolutionary process. Here, I use phylogenetic tools to show that evolution leaves figurative fingerprints on genomes over millions of years