Dong-Ho Kim

Heekyu Kim

Hojin Ryu

Jae Kook Lee

Keum-Il Jang

Seung Hee Eom

Tae Kyung Hyun

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EOM S. H., LEE J. K., KIM D.-H., KIM H., JANG K.-I., RYU H., HYUN T. K.1 ACTA BOT. CROAT. 77 (1), 2018On-line Suppl. Tab. 1. Primer sequences of Linum usitatissimum polyamine oxidase genes for qReal-time PCR analysis. Primer Sequences (5’-3’)LuPAO1-F GAGTCCCGGTCGATAACCTALuPAO1-Rev CAATCTTCAGCAGCCATCATLuPAO2-F CTCGCAAGAGATGTCCAGAALuPAO2-Rev AGGAACCAAGGGAGTTGATGLuPAO3-F AGAATGGGATTGAGGACCTGLuPAO3-Rev TTAGCCTCTTTACCGCCAATLuPAO4-F GCGTGGCTGTTACAGTGATTLuPAO4-Rev TACCAGCAAGGTATCCACCALuPAO5-F GCCGGAATGTCTGGAATATCLuPAO5-Rev GCTGAAATCGGTCTTCAACALuActin-F ACAGGGAGAAGATGACACAGLuActin-R ACCAGAATCCAGCACAATACOn-line Suppl. Fig. 1. Phylogenetic analysis of polyamine oxidase (PAO) family in flax and other plants. The genes and accession num-bers used were AtPAO1 (Arabidopsis thaliana PAO1, At5g13700); AtPAO2 (Arabidopsis thaliana PAO2, At2g43020); AtPAO3 (Ara-bidopsis thaliana PAO3, At3g59050); AtPAO4 (Arabidopsis thali-ana PAO4, At1g65840); AtPAO5 (Arabidopsis thaliana PAO5, At4g29720); OsPAO1 (Oryza sativa PAO1, NM_001050573); OsPAO2 (Oryza sativa PAO2, NM_001055782); OsPAO3 (Oryza sativa PAO3, NM_001060458); OsPAO4 (Oryza sativa PAO4, NM_001060753); OsPAO5 (Oryza sativa PAO5, NM_001060754); OsPAO6 (Oryza sativa PAO6, NM_001069545); OsPAO7 (Oryza sativa PAO7, NM_001069546); CsPAO1 (Citrus sinensis PAO1, Cs7g02060.1); CsPAO2 (Citrus sinensis PAO2, Cs7g18840.2); CsPAO3 (Citrus sinensis PAO3, Cs6g15870.1); CsPAO4 (Citrus sinensis PAO4, Cs4g141 50.1); CsPAO5 (Citrus sinensis PAO5, Cs7g23790.1); CsPAO6 (Citrus sinensis PAO6, Cs7g23670.1). I, II, III and IV indicate four major classes of polyamine oxidase family.On-line Suppl. Fig. 2. Multiple sequence alignment of Linum usitatissimum polyamine oxidase (LuPAO) C-terminal regions. Peroxisomal targeting signals of LuPAO1 and LuPAO2 are indicated in red.POLYAMINE OXIDASE GENES IN FLAXACTA BOT. CROAT. 77 (1), 2018 2On-line Suppl. Fig. 3. Putative motif distribution in Linum usitatissimum polyamine oxidase (LuPAO) proteins. Motifs of LuPAO proteins were investigated by MEME web server.On-line Suppl. Fig. 4. Putative cis-acting regulatory elements related to stress and hormone responses in promoter regions (5´ to 3´ direc-tion) of the Linum usitatissimum polyamine oxidase (LuPAO) gene family.

Identification and expression profiling of flax (Linum usitatissimum L.) polyamine oxidase genes in response to stimuli. On-line supplemnet

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