Anaphase kinetochore movements [1a. First place]

Abstract

In this HeLa cell, which is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin), one can visualize the dynamic nature of kinetochore movements during metaphase and anaphase. During metaphase sister kinetochore pairs, seen as paired dots oriented parallel to the x-axis, are attached to opposite spindle poles, marked by the two pairs of venus centrin dots near the left and right edges of the movie (seen as single dots in this movie). Kinetochores in metaphase HeLa cells typically undergo oscillatory movements around the equator of the spindle. At the onset of anaphase, the sisters separate and move poleward. As seen in this movie, however, kinetochores can continue to switch between poleward and away from pole movements even after sister separation. Note that the centrin dots are approximately 12 microns apart at the start of the movieComponente Curricular::Educação Superior::Ciências Biológicas::Biologia Gera