Kinetochores fail to align [1b. First place]

Abstract

This HeLa cell is expressing both a kinetochore marker (EGFP-CENP-B) and a centrosome marker (Venus-centrin) and was depleted of the mitotic kinesin, Kif18A. In control cells at this stage in mitosis, sister kinetochore pairs, seen as paired dots oriented parallel to the x-axis, are attached to opposite spindle poles, marked by the venus centrin dots near the left and right edges of the movie (usually seen as single dots in this movie), and typically undergo oscillatory movements around the equator of the spindleComponente Curricular::Educação Superior::Ciências Biológicas::Biologia Gera