The aim of study was to determine preliminary phytochemical analysis and the antioxidant potential of Ophiocordyceps sobolifera. The antioxidant activity of Ophiocordyceps sobolifera was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and total antioxidant activity methods. Phytochemical analyses revealed the presence of steroids, triterpenoids, alkaloids, phenolics, flavoinods, protein, carbohydarte and saponins. The antioxidant activity of the ethanol extracts and water extracts showed high antioxidant activity with the lowest half maximal inhibitory concentration (IC50) values to 0.70 from 0.95mg/mL, realtively. Total antioxidant capacity of the O. sobolifera showed contained from 5.52 ± 0.14 to 12,71 ± 0.23 mg GA/g or from 3.55 ± 0.15 to 7.87 ± .05 μmol AS/g. These data suggest that O. sobolifera is a natural source of antioxidants