This study examines organizational commitment concept and evaluates the gender factor as being an individual factor that was related with organizational commitment. As a result of the literature study and based on the conceptual background, it was suggested that gender would be a factor determining the organizational commitment. For examining the relationship between gender and organizational commitment, a research study was performed on the health care staff working in hospitals. The sample of the study was composed of the health care staff from two selected hospital in Istanbul. According to the results, a significant relationship between gender and organizational commitment was found. Besides, it was seen that there were significant relationships between the sub dimension of organizational commitment and gender factor. It was found that the dimension of organizational commitment differed in terms of the gender difference