A bryological inventory of the Upper Mazaruni District, Guyana (former British Guiana) yielded almost three hundred species, including 2 genera and 11 species new to science and 130 species new to the Guianas. The densely forested and very humid north slope of Mount Roraima (500-2300 m.) proved to be the richest area for bryophytes and most of the novelties were found there. The present paper provides an enumeration of the species collected with a brief characterization of their habitat. The following species are described as new: Haesselia acuminata Gradst., Plagiochila gymnocalyx Inoue, Radula gradsteinii Yamada, Radula guyanensis Yamada, Radula mazarunensis Yamada and Stenorrhipis grollei Gradst. Anastrophyllum subg. Vanaea Inoue & Gradst. from Mount Roraima is elevated to generic rank
