Chromosome numbers with information on meiotic behaviour are recorded for the first time for the following taxa in the family Orthotrichaceae from Papua New Guinea - Desmotheca apiculata (Dozy & Molk.) Lindb. ex Card. n=6; Macromitrium incurvifolium (Hook. & Grev.) Schwaegr. n=9, M. longicaule C. Muell. n=6 (5+X/y), M. orthostichum Nees ex Schwaegr. n=7 (6+ X/y), M. salakanum C. Muell. n=9, M. similirete Bartr. n=9. M. streimannii Vitt n=9 (8+m); Schlotheimia emarginato-pilosa Herz. n=9 and S. macgregorii Broth. & Geh. n=11