In the present study, we used a 16.4 T scanner to evaluate the quantification of metabolites from the rat brain with an ultra-short TE STEAM sequence. All metabolite resonances, previously observed in vivo and published in the literature, were detected. Additionally, a newly discernible Histidine peaks at 3.11 ppm, 3.22 ppm and 3.97 ppm were detected and quantified for the first time in vivo, with CRLB of below 20 , indicating high quantification reliability. These preliminary results demonstrate the feasibility of detecting new metabolites in combination with the advantages of a high magnetic field strength