Efficient interaction of light and matter at the ultimate limit of single photons and single emitters is of great interest from a fundamental point of view and for emerging applications in quantum engineering. However, the difficulty of generating single-photon streams with specific wavelengths, bandwidths, and power as well as the weak interaction probability of a single photon with an optical emitter pose a formidable challenge toward this goal. Here, we demonstrate a general approach based on the creation of single photons from a single emitter and their use for performing spectroscopy on a second emitter situated at a distance. While this first proof of principle realization uses organic molecules as emitters, the scheme is readily extendable to quantum dots and color centers. Our work ushers in a new line of experiments that provide access to the coherent and nonlinear couplings of few emitters and few propagating photons