Parallel-stranded oligonucleotides with alternating d(A◊isoG)/d(T◊C) and d(A◊G)/d(T◊m⁵isoG) sequences

Abstract

Parallel-stranded (ps) DNA hairpins with alternating d(A◊isoG)/d(T◊C) (designated as ps-t1) and d(A◊G)/d(T◊m5isoC) (ps-t2) sequences were studied by means of UV, CD and fluorescence spectroscopy. The thermostability of d(A◊G)/d(T◊m5isoC) sequence was close to that of aps d(G◊A)/d(T◊C). The stability of the ps d(A◊isoG)/d(T◊C) sequence was even higher than that of a related anti-parallel-stranded (aps) d(G◊A)/d(T◊C) sequence, being unique for ps DNAs studied so far