Alen

Buck

Colloms

D. J. Sherratt

Espeli

Gourlay

Grainge

I. Grainge

K. Ishihara

K. Shimokawa

M. Vazquez

Sadowski

Saka

Sumners

Valencia

Van Duyne

Vazquez

Significance
          
            Newly replicated circular chromosomes are topologically linked. XerC/XerD-
            dif
            (XerCD-
            dif
            )–FtsK recombination acts in the replication termination region of the
            Escherichia coli
            chromosome to remove links introduced during homologous recombination and replication, whereas Topoisomerase IV removes replication links only. Based on gel mobility patterns of the products of recombination, a stepwise unlinking pathway has been proposed. Here, we present a rigorous mathematical validation of this model, a significant advance over prior biological approaches. We show definitively that there is a unique shortest pathway of unlinking by XerCD-
            dif
            –FtsK that strictly reduces the complexity of the links at every step. We delineate the mechanism of action of the enzymes at each step along this pathway and provide a 3D interpretation of the results.
          </jats:p

Koya Shimokawa

Kai Ishihara

Ian Grainge

David J. Sherratt

Mariel Vazquez

Crossref

Proceedings of the National Academy of Sciences

FtsK-dependent XerCD-
            <i>dif</i>
            recombination unlinks replication catenanes in a stepwise manner

FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner

FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner

Abstract

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