Croatian Society of Food Technologists, Biotechnologists and Nutritionists
Abstract
U ovom istraživanju proveden je uzgoj alge Euglena gracilis s ciljem proizvodnje vitamina A i vitamina E. U svrhu određivanja najpovoljnijih parametara za proizvodnju vitamina u bioreaktoru s mješalom provedena je optimizacija sastava hranjive podloge i uvjeta uzgoja E. gracilis, u Erlenmeyer tikvicama na laboratorijskoj tresilici. Tijekom uzgoja alge E. gracilis u bioreaktoru s mješalom primijenjen je dvostupanjski uzgoj. U prvom stupnju proveden je fotoheterotrofni uzgoj na Hutner-ovoj podlozi, a u drugom stupnju miksotrofni uzgoj na podlozi s ograničenim izvorom ugljika. Primjena dvostupanjskog procesa osigurala je relativno visoku produktivnost proizvodnje vitamina A (PrA = 0,07 mg/Lh) i vitamina E (PrE= 0,27 mg/Lh), a prinosi su iznosili vitamina A (YA = 6,53 mg/L) odnosno vitamina E (YE = 26,26 mg/L). Visoki prinosi vitamina E ukazuju na mogućnost primjene dvostupanjskog procesa njegove proizvodnje u industrijskom mjerilu.In this research, cultivation of algae Euglena gracilis was studied in order to produce vitamin A and vitamin E. The optimization of medium composition and cultivation conditions, in Erlenmeyer flasks on laboratory shaker, was done to define the most appropriate parameters for vitamin production in the stirred tank bioreactor. During cultivation of E. gracilis in the stirred tank bioreactor two-step process was examined. In the first step photoheterotrofic cultivation on the Hutner medium was done and in the second step photomixotrophically cultivation on the limited carbon source medium. In the two step process following bioprocess efficiency parameters were observed: productivity for vitamine A (PrA =
0.07 mg/Lh) and vitamin E (PrE= 0.27 mg/Lh) production as well as vitamin A (YA = 6.53 mg/L) and vitamin E (YE = 26.26 mg/L) yield. High vitamin E yield indicate potential of two-step processes for this vitamin industrial scale production
