Faculty of Veterinary Medicine University of Zagreb
Abstract
Experiments were performed to determine whether the nematode -trapping fungus Monacrosporium thaumasium is able to survive encapsulation in sodium alginate and the effects of different temperatures and mineral salt. Pellets of sodium alginate were treated with paraffin, mineral salt or without these elements. They were placed in 250 ml Erlenmeyer flasks at 4 °C, room temperature, 25 °C, 30 °C and 35 °C. Over a 16-week period, once a week one pellet was placed in the center of an 8.5 cm Petri dish containing 20 ml of 2% Potato Dextrose agar, and radial growth was monitored for nine days. The best treatment (P<0.01) involved fungal pellets without paraffin, at 4 °C, and which remained viable for up to 16 weeks of storage. Pellets without paraffin induced higher growth than pellets with paraffin at room temperature, as well as pellets without mineral salt in all temperatures (P<0.01). This formulation of the fungus proved to be a powerful tool for use as biological control of nematodes and will be limited by storage conditions.Pokusi su provedeni da se utvrdi da li Monacrosporium thaumasium, gljivica koja napada obliće može preživjeti svoju inkapsulaciju u natrijevom alginatu pri različitim temperaturama i uz mineralne soli. Pelete natrijevog alginata bile su podvrgnute različitim temperaturama, i to bez prethodne obrade, ili nakon obrade parafinom ili mineralnom soli. U Erlenmajerovim posudicama od 250 ml držane su na 4 °C, na sobnoj tempereaturi, na 25 °C, 30 °C i 35 °C. Tijekom 16 tjedana, jednom tjedno je po jedna peleta stavljana u sredinu Petrijeve posudice s 20 ml 2% agara od krumpirove dekstroze, te je promatran njen radijalni rast tijekom 9 dana. Najbolji rast (P<0.01) pokazale su gljivične pelete bez obrade parafinom i držane na 4 °C, koje su sačuvale sposobnost rasta do 16 tjedana pohranjivanja. Pelete bez parafina su i na sobnoj temperaturi pokazivale bolji rast od onih obra|enih parafinom, kao što su bolje rasle i pelete bez mineralne soli na svim temperaturama (P<0.01). Ovakva primjena gljivica se pokazala kao moćno sredstvo biološke kontrole nametničkih oblića, ali je njegova uporaba ograničena uvjetima pohranjivanja
