U ovom radu istražena je osjetljivost koagulaza-negativnih stafilokoka (n=78) i bakterija mliječne kiseline (n=30) na klindamicin, tetraciklin, amikacin, amoksicilin + klavulanska kiselina, enrofloksacin, vankomicin, trimetoprim + sulfametoksazol, tobramicin, kloramfenikol, ciprofloksacin, eritromicin, penicilin i trimetoprim primjenom disk-difuzijskog testa i/ili E-testa. Izolati su izdvojeni iz industrijski proizvedenih mekih i tvrdih sireva, maslaca i salamure. Svi izolati stafilokoka bili su osjetljivi na klindamicin, amikacin, amoksicilin + klavulansku kiselinu, enrofloksacin, vankomicin, kloramfenikol i ciprofloksacin prema CLSI (Clinical and Laboratory Standards Institute) kriterijima. Ukupno 30 izolata (38,46 %) stafilokoka bilo je otporno na eritromicin, 18 na penicilin (23,07 %), 4 na tetraciklin (5,12 %), te po jedan izolat na trimetoprim, tobramicin i trimetoprim + sulfametoksazol (1,28 %). Od ukupno 78 testiranih izolata stafilokoka, njih 35 bilo je rezistentno na najmanje jednu antimikrobnu tvar (44,87 % izolata). Udio rezistentnih izolata u mekim sirevima kretao se od 22 do 70 % testiranih stafilokoka, dok u tvrdom siru i salamuri rezistentnih izolata nije bilo. Na rast bakterija mliječne kiseline nisu utjecali trimetoprim + sulfametoksazol (n=29), vankomicin (n=29), trimetoprim (n=28), amikacin (n=10) i tobramicin (n=10). Dobiveni rezultati ukazuju na to da značajan udio nepatogene mikroflore različitih mliječnih proizvoda s hrvatskog tržišta pokazuje fenotipsku rezistenciju na antimikrobne tvari.In this research, the susceptibility to clindamycin, tetracycline, amikacin, amoxicillin + clavulanic acid, enrofloxacine, vancomycin, trimethoprim + sulphametoxazol, tobramycin, chloramphenicol, ciprofloxacin, erythromycin, penicillin and trimethoprim was tested in coagulase-negative staphylococci (n=78) and lactic acid bacteria (n=30) by means of disk diffusion test and E-test. The isolates were collected from soft and hard cheeses, butter and brine. All isolates of coagulase-negative staphylococci were susceptible to clindamycin, amikacin, amoxicillin + clavulanic acid, enrofloxacine, vancomycin, chloramphenicol and ciprofloxacin according to CLSI breakpoints. A total of 30 staphylococci isolates (38.46 %) were resistant to erythromycin, 18 to penicillin (23.07 %), 4 to tetracycline (5.12 %), and one isolate to trimethoprim, tobramicin and trimethoprim + sulphametoxazol (1.28 %). Among 78 tested staphylococci, 35 of them were resistant to at least one antimicrobial substance (44.87 %). The rate of resistant isolates of different soft cheese types ranged from 22 to 70 %, while resistant staphylococci were absent in hard cheese and brine. The growth of lactic acid bacteria was not influenced by trimethoprim + sulphametoxazol (n=29), vancomycin (n=29), trimethoprim (n=28), amikacin (n=10) and tobramycin (n=10). The results show that significant part of apathogenic microbiota in different dairy products is phenotypically resistant to antimicrobial agents
