A field experiment was conducted with 22 Brassica napus L. advanced lines at the experimental farm of Sher-e-Bangla Agricultural University, Dhaka from November 2008 to March 2009 to asses genetic diversity among advanced lines of Brassica napus L. Different multivariate analysis techniques were used to classify 22 Brassica napus genotypes. The genotypes were grouped into four clusters. The highest inter-cluster distance was observed between clusters II and IV whereas the maximum intra-cluster distance was found in cluster II. Therefore, the genotypes belonging to cluster I and cluster II, cluster II and cluster III and cluster III and cluster IV have been selected for future hybridization program. The role of number of secondary branches per plant and number of siliqua per plant in both vectors were important components for genetic divergence in these materials. Considering group distance and other agronomic performance the inter-genotypic crosses between G1 and G2, G2 and G6; G6 and G7; G6 and G8 and G7 and G8 might be suggested for future hybridization program