Faculty of Veterinary Medicine University of Zagreb
Abstract
The reverse transcriptase-polymerase chain reaction/restriction fragment length polymorphism (RTPCR/ RFLP) and RT-PCR specific primer to detect very virulent (vv) strains of infectious bursal disease virus (IBDV) were used for identification and characterization of Jordanian field isolates of IBDV that caused severe outbreaks. In this study, 80 bursa of fabricius samples were used from 20 commercial broiler chicken flocks in Jordan with clinical symptoms of IBDV. The RT-PCR/RFLP was conducted on a 743-bp fragment of the VP2 gene with the restriction enzymes BstNI and MboI. The results indicate the existence of IBDV field strains in Jordan. In addition, 60% of IBDV Jordanian isolates had unique RFLP patterns different from those previously published elsewhere. However, 20% of local IBDV strains were positive on using a specific primer for vvIBDV and had a unique RFLP pattern that differed from the Jordanian IBDV isolate and those previously published elsewhere.Lančana reakcija polimerazom uz prethodnu reverznu transkripciju/polimorfizam dužine restrikcijskih fragmenata (RT PCR/RFLP) i RT-PCR specifične početnice za jako virulentne sojeve virusa zarazne bolesti Fabricijeve burze (ZBFB) bile su rabljene za identifikaciju i karakterizaciju jordanskih terenskih izolata toga virusa koji su uzrokovali teški oblik bolesti. Za istraživanje je bilo rabljeno 80 uzoraka tkiva Fabricijeve burze uzetih iz 20 komercijalnih jata tovnih pilića u Jordanu u kojih su ustanovljeni klinički znakovi ZBFB a. RT-PCR/ RFLP proveden je na odsječku gena VP2 od 743-bp s restrikcijskim enzimima BstNI i MboI. Rezultati upućuju na postojanje terenskih sojeva virusa ZBFB-a od kojih je 60% izolata imalo jedinstveni RFLP obrazac različit od onoga već objavljenog drugdje. Ipak, 20% lokalnih sojeva virusa bilo je pozitivno uporabom specifične početnice za jako virulentni virus i imalo je jedinstveni obrazac RFLP različit u jordanskih izolata u odnosu na izolate objavljene drugdje
