Faculty of Veterinary Medicine University of Zagreb
Abstract
For the purpose of investigating the effects of iron supplementation on the therapy of experimental canine ancylostomosis, twenty 8 week old pups were rando mly divided into four equal groups A, B, C and D. Groups A, B and C were serially infected bi-weekly with infective Ancylostoma caninum (L3) larvae up to 98 days. Thereafter, Groups A and B were treated with pyrantel pamoate and dextrose saline infusion with or without iron dextran supplementation, respectively. Pups in Group C were left untreated as were the uninfected control pups in Group D. Clinical observations revealed a serial reversion of the distressing hookworm disease signs in only the two infected and treated Groups A and B, but not in the infected but untreated pups in Group C. Both the haematological, and immunological parameters were most elevated in the iron-treated pups in Group A, with high and positive correlation coefficient (r) of + 0.94, 0.83, 0.41 and 0.98 for the respective Groups A, B, C and D. Iron therapy was suggested as being responsible for the prompt haematological and immunological reversion observed in Group A pups through its facilitation of the haemoglobin synthesis and the iron dependent mitotic cell division processes.U pokusu određivanja učinka dodatka željeza na terapiju pokusno izazvane ankilostomoze pasa, 20 štenadi u dobi 8 tj. bilo je podijeljeno u četiri skupine označene A, B, C i D. Skupine A, B i C bile su svaka dva tjedna invadirane invazijskim ličinkama Ancylostoma caninum (ličinke 3. stupnja) u razdoblju 98 dana. Nakon invazije skupine A i B bile su liječene pirantel pamoatom i infuzijama fiziološke otopine dekstroze s dodatkom ili bez dodatka željeza i dekstrana. Skupina C i kontrolna skupina D nisu bile liječene. Ustanovljeno je znatno poboljšanje kliničkih znakova bolesti u štenadi skupine A i B, ali ne i u invadirane i neliječene štenadi skupine C. Vrijednosti imunoloških i hematoloških pokazatelja bile su mnogo više u skupini A liječenoj željezom s visokim i pozitivnim korelacijskim koeficijentom (r) od +0,94, 0,83, 0,41 i 0,98 za odgovarajuće skupine A, B, C i D. Smatra se da je terapija željezom, putem olakšane sinteze hemoglobina i o željezu ovisnih procesa mitoze, dovela do brzog poboljšanja hematoloških i imunoloških pokazatelja u štenadi skupine A
