POJAVA PHOMOPSIS SP. NA ACHILLEA MILLEFOLIUM

Abstract

During a three year period (2004 to 2006) on locations of Eastern Croatia (Kneževi Vinogradi, Osijek, Brođanci and Krndija) we collected plants of Achillea millefolium L. from which we isolated Phomopsis sp. Many of the collected plants exhibited no visible symptoms of infection but in spring pycnidia were noticed on overwintered plants. Isolation was done on PDA from naturally infected plants and morphological and molecular characteristics were studied. Biometrical values of reproductive structures developed on naturally infected plants and grown on PDA were measured. Internal transcribed spacers ITS rDNA of the examined isolates were sequenced. Although molecular analysis showed that isolates were grouped with Diaporthe arctii, we decided to classify isolates from A. millefolium as Phomopsis sp., the details of which were discussed in the paper.Phomopsis sp. izolirali smo s biljaka A. millefolium na kojima nije bilo vidljivih simptoma zaraze, kao i s biljaka sa simptomima tipičnima za Phomopsis vrste. Biljke su prikupljene tijekom tri godine (2004.-2006. godine) u jesen i proljeće na lokacijama (Kneževi Vinogradi, Osijek, Brođanci i Krndija) istočne Hrvatske. S prirodno zaraženih biljaka izolacija gljive obavljena je na KDA te su proučene morfološke i molekularne karakteristike. Biometrijske vrijednosti reproduktivnih struktura izmjerene su u kulturi (KDA) i na prirodno inficiranome materijalu. Za proučavane izolate sekvencirane su unutarnje prijepisne razmaknice jezgrine DNA (ITS rDNA) te je utvrđena povezanost analiziranih izolata s Diaporthe arctii. Zbog nedovoljno pouzdanih informacija zadržan je naziv Phomopsis sp. detalji su raspravljeni u radu