A Swidsinski

C Walton

CE Garner

Christian Ludwig

DC Baumgart

DL Topping

DM Jacobs

DN Frank

E Ricart

F Dong

F Sipos

F Wu

Fuhua Hao

G Gall Le

H Duboc

H Hove

H Sokol

H-M Lin

HRT Williams

I Ahmed

I Ordás

J Jansson

J Olsen

J Saric

J Trygg

J Vermeiren

Jacob Tveiten Bjerrum

JP Segain

JR Marchesi

JT Bjerrum

K Balasubramanian

K Geboes

KW Schroeder

L Eriksson

M Bylesjö

M Martinez-Medina

M Ooi

MA Chapman

Mehmet Coskun

MJ Etchevers

N Planell

NS Stephens

O Cloarec

Ole Haagen Nielsen

P Stein von

P Vernia

R Schicho

R Thibault

RB Sartor

RF Harvey

S Nikolaus

S Tedelind

SH Duncan

SR Vavricka

T Bezabeh

T Dawiskiba

TB Murdoch

Ulrich Günther

V Preter De

VK Tso

Y Zhang

Yulan Wang

English

Abstract
          This study employs spectroscopy-based metabolic profiling of fecal extracts from healthy subjects and patients with active or inactive ulcerative colitis (UC) and Crohn’s disease (CD) to substantiate the potential use of spectroscopy as a non-invasive diagnostic tool and to characterize the fecal metabolome in inflammatory bowel disease (IBD). Stool samples from 113 individuals (UC 48, CD 44, controls 21) were analyzed by 1H nuclear magnetic resonance (NMR) spectroscopy (Bruker 600 MHz, Bruker BioSpin, Rheinstetten, Germany). Data were analyzed with principal component analysis and orthogonal-projection to latent structure-discriminant analysis using SIMCA-P + 12 and MATLAB. Significant differences were found in the metabolic profiles making it possible to differentiate between active IBD and controls and between UC and CD. The metabolites holding differential power primarily belonged to a range of amino acids, microbiota-related short chain fatty acids, and lactate suggestive of an inflammation-driven malabsorption and dysbiosis of the normal bacterial ecology. However, removal of patients with intestinal surgery and anti-TNF-α antibody treatment eliminated the discriminative power regarding UC versus CD. This study consequently demonstrates that 1H NMR spectroscopy of fecal extracts is a potential non-invasive diagnostic tool and able to characterize the inflammation-driven changes in the metabolic profiles related to malabsorption and dysbiosis. Intestinal surgery and medication are to be accounted for in future studies, as it seems to be factors of importance in the discriminative process.</jats:p

Crossref

Metabolomics

Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn’s disease and healthy individuals

https://link.springer.com/content/pdf/10.1007%2Fs11306-014-0677-3.pdf

Metabonomics of human fecal extracts characterize ulcerative colitis, Crohn’s disease and healthy individuals

Abstract

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