Faculty of Veterinary Medicine University of Zagreb
Abstract
Field samples were identified by agar gel precipitation and polymerase chain reaction (PCR) as positive for sheeppox virus. Envelope protein gene PCR amplicons (192 bp) of Indian sheeppox viruses isolated in 1997 and 2003 were sequenced to analyse nucleotide divergence. Analysis revealed that 2003 isolates possessed 100% nucleotide identity to each other, but only 95% nucleotide and amino acid were identity to an isolate from 1997. These Indian isolates were unique from other capripox virus sequences in that they contained a single codon insertion. Consistent with previous findings, the results indicate that recent sheepox virus isolates in India are more similar to previously described sheepox virus than to goatpox viruses from India and elsewhere.Virus ovčjih boginja dokazan je u terenskim uzorcima imunodifuzijom u gelu i lančanom reakcijom polimeraze (PCR). PCR umnošci gena za protein ovojnice (192 pb) indijskih izolata virusa ovčjih boginja izdvojenih tijekom 1997. i 2003. razlikovali su se po nukleotidnom slijedu. Izolati iz 2003. međusobno su bili identični u 100% nukleotida, dok je izolat iz 1997. bio njima podudaran samo u 95% nukleotida i aminokiselina. Ti indijski izolati bili su jedinstveni u odnosu na druge viruse boginja koza po jednom ugrađenom kodonu. U skladu s prijašnjim istraživanjima, nalazi ukazuju da su nedavni izolati virusa ovčjih boginja u Indiji sličniji ranije opisanim virusima ovčjih boginja nego virusima kozjih boginja iz Indije i drugdje
