Utjecaj davanja maslaca obogaćenoga konjugiranom linolnom kiselinom na incidenciju i patohistološke promjene raka mliječne žlijezde štakorica uzrokovanoga 7,12-dimetilbenz[a]anthracenom.

Abstract

A study was conducted to investigate the influence of Conjugated Linoleic Acid (CLA) feeding on cancer incidence and histopathological changes in DMBA induced mammary gland carcinogenesis rats. Female Wistar rats of 21 days were taken for the study and the animals were randomly divided into three groups of 30 animals each. Group I animals were fed with a soybean based diet, whereas group II and group III animals were fed with a low CLA and high CLA ghee based diet, respectively. Soybean oil, low CLA and high CLA ghee were included at the level of 20 per cent in the animal diet. Feeding with test diets started on the day of weaning. The animals were given 7,12 dimethyl-benz[a]anthracene(DMBA) at 5 mg per animal as a single dose by oral intubation at the age of 55 days and the test diets were continued up to 32 weeks after DMBA administration. At the end of 32 weeks all the animals were sacrificed, tumour data recorded and histopathology performed. The tumour incidence in groups I, II and III was 83.33, 63.33 and 46.07 per cent respectively. Histopathology revealed fibroma, adenoma, fibroadenoma in all the groups whereas in group I showed adenocarcinoma. Therefore, the results of the present study showed CLA not only inhibited benign type tumours but malignant tumours as well.Istraživan je utjecaj davanja maslaca od bivoljega mlijeka obogaćenoga konjugiranom linolnom kiselinom na incidenciju i patohistološke promjene u nastanku raka mliječne žlijezde štakorica uzrokovanoga 7,12-dimetilbenz[a]antracenom. Wistar štakorice u dobi od 21 dana bile su nasumce podijeljene u tri skupine po 30 životinja. Prva skupina bila je hranjena hranom na bazi soje, dok je druga dobivala hranu na bazi bivoljega mlijeka s malom koncentracijom konjugirane linolne kiseline, a treća hranu s dodatkom veće količine konjugirane linolne kiseline. Sojino ulje i linolna kiselina dodavani su u hranu u količini od 20%. Hranidba navedenom hranom započela je na dan zalučenja. Svakoj je životinji peroralno dan 7,12-dimetilbenz[a]antracen (DMBA) u količini od 5 mg u dobi od 55 dana, a testirana hrana davana je do 32 tjedna nakon primjene DMBA. Sve su životinje bile žrvovane nakon 32 tjedna te pretražene na pojavnost i patohistološke značajke tumora. Incidencija tumora u I skupini bila je 83,33%, u II skupini 63,33% i u III skupini 46,07%. Patohistološki je ustanovljen fibrom, adenom i fibroadenom u štakorica svih skupina, dok je u štakorica I skupine ustanovljen i adenokarcinom. Rezultati su pokazali da je konjugirana linolna kiselina imala inhibirajući učinak i na dobroćudni i na zloćudni tumor