Whey beverage was prepared by utilizing Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 and Propionibacterium freudenreichii subsp. shermanii MTCC 1371 in order to make a fermented probiotic healthy drink. The product made with 4 % mixed culture (1:1:1) inoculated (initial count - lactobacilli 6.2 x 107 CFU/mL, bifidobacteria 5.4 x 107 CFU/mL, propionibacteria 3.9 x 107 CFU/mL) in deprotienized whey (4.6 % lactose, 0.62 % ash, 0.48 % fat and 0.5 % protein) adjusted to pH 6.4 and incubated at 37 °C for 8 h has a good technological and dietetic criteria required for a probiotic product. Total bacterial count, lactobacilli count, bifidobacteria count, propionibacteria count, titratable acidity, β-D galactosidase activity, concentration of lactic acid and sensory properties were monitored during storage period. The whey beverage fermented for 8 h and prepared with 4 % inoculum of mixed culture (1:1:1) met the probiotic criterion by maintaining each type of bacterial population at counts greater than 108 CFU/mL up to 10 days of storage period. The titratable acidity as well as sensory properties did not change appreciably during first 7 days of storage. At the end of 15 days of storage, slight acidification was detected, although the beverage still retained an acceptable flavour.Napitak sirutke je pripremljen korištenjem Lactobacillus rhamnosus NCDO 243, Bifidobacterium bifidum NCDO 2715 i Propionibacterium freudenreichii subsp. shermanii MTCC 1371 u svrhu pripravljanja probiotskog zdravog napitka. Proizvod je pripravljen s 4 % inokuluma mješovite kulture (1:1:1, početni broj - laktobacili 6,2 x 107 CFU/mL, bifidobacteria 5,4 x 107 CFU/mL, propionibacteria 3,9 x 107 CFU/mL) u deproteiniziranoj sirutki (4,6 % laktoze, 0,62 % pepela, 0,48 % masti i 0,5 % proteina) prilagođenog pH (6,4) i inkubiranog na 37 °C kroz 8 h radi postizanja dobrih tehnoloških i dijetetskih kriterija za dobivanje probiotičkog proizvoda. Tijekom skladištenja promatrani su ukupni broj bakterija, broj laktobacila, broj bifidobakterija, koncentracija mliječne kiseline te senzorska svojstva. Sirutka fermentirana s 4 % inokuluma mješovite kulture tijekom 8 sati postigla je kriterije zadane za probiotičke napitke, s brojem svakog pojedinog soja većim od 108 CFU/mL tijekom 10 dana skladištenja. Titracijska kiselost i senzorska svojstva nisu se značajno mijenjala tijekom 7 dana skladištenja. Na kraju 15-tog dana skladištenja, pojavila se blaga kiselost iako je napitak još uvijek bio ocijenjen kao prihvatljiv obzirom na okus
