SAŽETAK. Cilj: Za koštane morfogenetske proteine -2 i -7 pokazano je da osim snažnog
osteoinduktivnog svojstva imaju važnu ulogu u razvoju i cijeljenju različitih neskeletnih tkiva i
organa. Svrha ovog rada je ispitati ekspresiju BMP-2 i BMP-7 tijekom upalne bolesti crijeva u
štakora, te učinak dvotjednog liječenja sa BMP-7 na cijeljenje tkiva kolona i ekspresiju ovih
morfogenetskih proteina. Metode: Eksperimentalni kolitis izazvan je primjenom klizme koja sadrži
2,4,6-trinitrobenzensulfoničnu kiselinu (TNBS) i etanol. Na uzorcima crijeva pratili smo
makroskopske i mikroskopske promjene koje se zbivaju tijekom akutne i kronične faze kolitisa, te
nakon i.v. primjene BMP-7. Metodom imunohistokemije i lančane reakcije polimeraze pratili smo
izražaj BMP-2 i -7. Rezultati: Imunohistokemijska i RT-PCR analiza pokazala je ekspresiju BMP-2 i -7
tijekom akutne i kronične faze upalne bolesti crijeva. U akutnoj fazi kolitisa BMP-2 i -7 prisutni su u
području ulkusa, a u kroničnoj fazi BMP-7 pozitivno bojanje prisutno je u kolonocitima uzduž kripti
kolona, dok je BMP-2 pozitivno bojanje u kolonocitima površnog epitela te između kripta.
Specifično bojanje na BMP-7 kao i ekspresija mRNA za BMP-7 na uzorcima crijeva životinja liječenim
sa BMP-7 pokazuje smanjenje prisustva BMP-7, a pojačano bojanje i ekspresiju mRNA za BMP-2.
Zaključak: Sistemska primjena koštanog morfogenetskog proteina-7 smanjuje makroskopsko i
mikroskopsko oštećenje kolona tijekom upalne bolesti crijeva u štakora. Način ekspresije BMP-2 i
-7 tijekom upalne bolesti crijeva te nakon liječenja sa BMP-7 ukazuje na značajnu ulogu koštanih
morfogenetskih proteina u kontroli oštećenja i cijeljenja tkiva kolona tijekom ove bolesti.ABSTRACT. Aim: Bone morphogenetic proteins -2 and -7, along with their osteoinductive
function, play important role in the development and healing of different non-skeletal tissues
and organs. The aim of this study was to reveal the expression of BMP-2 and BMP-7 during
experimental inflammatory bowel disease, as well as after 14th day of BMP-7 treatment.
Methods: Experimental colitis was induced by the intracolonic administration of the clysma
containing 2,4,6-trinitrobenzensulphonic acid (TNBS) and ethanol. We investigated the
macroscopic and microscopic colon tissue damage during acute and chronic phases of colitis as
well as after IV treatment of BMP-7. Results: Imunohistochemical and RT-PCR analyses showed
expression of BMP-2 and -7 during acute and chronic phases of inflammatory bowel disease. In
the acute phase of colitis, BMP-2 and -7 expressions were located in mucosa of ulcer region. In
the chronic phase BMP-7 expression was placed in the colonocytes along the crypt, while BMP-2
expression was located in the coloncytes of surface epithelium and intercrypt table. BMP-7
treatment reduced the expression of BMP-7 and mRNA for BMP-7 but also increased the
expression of BMP-2 and mRNA for BMP-2 in colonic tissue. Conclusions: Systemic administration
of bone morphogenetic protein 7 decreased macroscopic and microscopic colonic tissue damage
during experimental inflammatory bowel disease. The expression pattern of BMP-2 and -7 during
inflammatory bowel disease, as well as after BMP-7 treatment suggests an important role of
bone morphogenetic proteins in control of colon tissue damage and healing during disease