Faculty of Veterinary Medicine University of Zagreb
Abstract
Blood lipid peroxides (TBARS) and naturally present blood antioxidants glutathione (GSH), haemoglobin (Hb) and plasma albumin (ALB) were determined in nine Simmental young bulls. Blood samples were taken five times. The animals were intensively fattened. Mean TBARS content showed a biphasic pattern in the course of growth with the highest value at 11.7 months of age. GSH content increased until the age of 8 months when the highest concentration was determined. Albumin and Hb concentrations were highest at 15.2 months of age. The main contributor to the significant increase and decrease of TBARS values could be the development of skeletal muscle tissue. Correlations among the parameters investigated were not significant in the course of growth, with the exception of GSH vice Hb at 5.4 months of age and TBARS vice Hb and TBARS vice ALB at 15.2 months of age. It might be concluded that the indicators of oxidant stress in young bulls were within the physiological range and that, possibly, blood natural antioxidants complemented each other.U punoj krvi devet simentalskih bičića određivani su lipidni peroksidi (TBARS), prirodni antioksidansi glutation (GSH) i hemoglobin (Hb), te albumin u plazmi. Životinje su bile u intenzivnom tovu. Prosječna koncentracija TBARS pokazivala je tijekom tova bifazno kretanje s najvišom vrijednosti u dobi životinja od 11,7 mjeseci. Koncentracija GSH je rasla do dobi od 8 mjeseci kada je utvrđena najviša vrijednost. Koncentracije albumina i Hb bile su najviše u dobi od 15,2 mjeseca. Pretpostavlja se da je razvoj i rast skeletnih mišića znatno pridonio signifikantnom porastu i smanjenju TBARS vrijednosti. Korelacije među istraživanim pokazateljima nisu bile signifikantne s izuzetcima GSH i Hb u dobi od 5,4 mjeseca, te TBARS i Hb uz TBARS i albumin u dobi životinja od 15,2 mjeseca. Zaključuje se da su se određivane vrijednosti pokazatelja oksidativnog stresa u bičića kretale unutar fizioloških granica, te da se prirodni antioksidansi u krvi nadopunjuju u djelovanju tijekom tova
