Faculty of Food Technology and Biotechnology, University of Zagreb
Abstract
Over the last decade there has been a significant interest in developing biotechnological applications of tyrosinases. These applications include the production of L-DOPA (3,4-dihydroxyphenyl-L-alanine) from L-tyrosine, the production of cross-linked protein networks for use as novel food additives and the detection of phenolic compounds in wastewater or their removal from it. Much of the research into these applications has involved mushroom tyrosinases. We review the potential biotechnological applications of mushroom tyrosinases and evaluate the state of knowledge about their production, recovery and immobilization. We conclude that much more research is necessary in these areas if mushroom tyrosinases are to fulfill their biotechnological potential.U posljednjem se desetljeću povećalo zanimanje za primjenu tirozinaze u biotehnologiji. Primjena obuhvaća proizvodnju L-DOPA (3,4-dihidroksifenil-L-alanin) iz L-tirozina, proizvodnju umreženih polimera za dodatak hrani te određivanje ili uklanjanje fenolnih spojeva iz otpadnih voda. Veći dio tih istraživanja odnosi se na tirozinazu gljiva. Prikazana je mogućnost primjene tirozinaze gljiva u biotehnologiji i ocijenjene dosadašnje spoznaje o njihovoj proizvodnji, izdvajanju i imobiliziranju. Zaključeno je da je potrebno provesti dodatna istraživanja kako bi se povećala mogućnost primjene tirozinaze gljiva u biotehnologiji
