Cilj ovog rada bio je istražiti utjecaj probiotičkog soja Bifidobacterium animalis subsp. lactis LAFTI® B94, inulina i transglutaminaze na kvalitetu i senzorska svojstva čvrstog jogurta. Za proizvodnju jogurta korišteno je svježe homogenizirano mlijeko s 3,3% mliječne masti kojemu je, uz jogurtne starter kulture, dodana probiotička kultura Bifidobacterium animalis subsp. lactis LAFTI® B94, inulin i enzim transglutaminaza, aktiviran 1 h i 30 min. pri 55 °C. Inaktivacija enzima provedena je postupkom pasterizacije 15 min. pri 85 °C. Proizvedeni su i kontrolni uzorci čvrstog jogurta, bez dodatka probiotičke kulture, inulina i transglutaminaze. U radu su određivana fizikalnokemijska i senzorska svojstva proizvedenih čvrstih jogurta. Radi pouzdane identifikacije probiotičkog soja Bifidobacterium animalis subsp. lactis LAFTI® B94, izolira-nog iz proizvedenog jogurta, provedena je SDS-poliakrilamidna gel elektroforeza (SDS-PAGE) ukupnih staničnih proteina i lančana reakcija polimera-zom (PCR) s početnicama specifičnim za rod Bifidobacterium. Proizvedeni čvrsti jogurti s dodanom probiotičkom kulturom, inulinom i transglutami-nazom su bili čvršći i pokazali su manju sinerezu, te su dobili više ocjene senzorskih svojstava u odnosu na kontrolne uzorke jogurta. Broj živih stanica Bifidobacterium animalis subsp. lactis LAFTI® B94 nakon 28 dana čuvanja bio je viši u uzorcima jogurta koji su sadržavali inulin kao prebiotik. Fermentacija jogurta s inulinom i transglutaminazom je kraće trajala od fermentacije jogurta bez dodatka inulina i transglutaminaze. SDS poliakrilamidnom gel elektroforezom ukupnih staničnih proteina i lančanom reakcijom polimeraze, primjenom početnica specifičnih za rod Bifidobacterium, potvrđena je prisutnost probiotičke kulture u proizvedenom čvrstom jogurtu u visokom broju (više od 106 stanica/mL).The aim of this research was to examine the influence of probiotic strain Bifidobacterium animalis subsp. lactis LAFTI® B94, inulin and transglutaminase on quality and sensory properties of set-style yoghurt. Fresh, homogenized milk with 3,3% of milk fat was used for yoghurt production, with addition of Bifidobacterium animalis subsp. lactis LAFTI® B94, inulin and transglutaminase activated during 1h 30 min at 55 °C. Enzyme inactivation was carried out by pasteurization of milk during 15 minutes at 85 °C. Control samples were prepared without addition of probiotic culture, inulin and transglutaminase. Physico-chemical parameters and sensory properties of produced set-style yoghurt have been determined. For reliable identification of probiotic strain Bifidobacterium animalis subsp. lactis LAFTI® B94, isolated from the produced yoghurt, SDS-PAGE of whole cell proteins and PCR with species specific primers for Bifidobacterium were carried out. It has been shown that produced set-style yoghurt with probiotic strain Bifidobacterium animalis subsp. lactis LAFTI® B94, inulin and transglutaminase had higher firmness, less syneresis and better sensory properties than control yoghurt samples. After 28 days of storage the viable count of Bifidobacterium animalis subsp. lactis LAFTI® B94 was higher in samples containing inulin as prebiotic. Fermentation of yoghurt samples containing inulin and transglutaminase lasted shorter than fermentation of samples without inulin and transglutaminase. The presence of high number of probiotic culture (more than 106 cells/mL) in produced set yoghurts was confirmed by SDS-PAGE of whole cell proteins and PCR with species specific primers for Bifidobacterium
