Faculty of Food Technology and Biotechnology, University of Zagreb
Abstract
Microwave-assisted extraction (MAE) technique in combination with multi-stage countercurrent extraction (MCE), namely microwave multi-stage countercurrent extraction (MMCE), was evaluated for the extraction of dihydromyricetin (DMY) from Ampelopsis grossedentata. Ethanol, methanol and water were used as extract solvents in the MMCE method. Of the three solvents used, water was found to be the best in extracting DMY from Ampelopsis grossedentata because it had a good extraction yield and is inexpensive, non-toxic and environmentally friendly. The optimal conditions of MMCE for the extraction of DMY can be determined to be the ratio of the extraction solvent to plant material of 30:1, the extraction time of 5 min, the extraction temperature of 110 °C and the microwave power of 600 W. In addition, the extraction efficiency of the MMCE method was compared with that of the microwave static batch extraction (MSBE) under the optimum extraction conditions. It was found that the MMCE method offered higher extraction efficiency than the MSBE method. Thus, the study suggests that the MMCE method provides an alternative technique in terms of both cost and efficiency.Ekstrakcija dihidromiricetina iz lišća biljke Ampelopsis grossedentata pokusno je provedena metodom mikrovalne ekstrakcije u kombinaciji s višefaznom protustrujnom ekstrakcijom. Kao otapalo upotrijebljeni su etanol, metanol i voda. Voda je najbolja za ekstrakciju dihidromiricetina iz Ampelopsis grossedentata jer daje dobro iskorištenje, jeftina je, nije toksična i ekološki je prihvatljiva. Kao optimalni uvjeti ovako kombinirane metode za ekstrakciju dihidromiricetina određeni su: omjer otapala i biljnog materijala 30:1, vrijeme ekstrakcije od 5 min, temperatura od 110 °C i snaga mikrovalova od 600 W. Uspoređujući učinkovitost ove metode s metodom diskontinuirane mikrovalne ekstrakcije u optimalnim
uvjetima, vidi se da se veća učinkovitost ekstrakcije postiže višefaznom nego diskontinuiranom mikrovalnom ekstrakcijom. Stoga se, s obzirom na troškove i njezinu učinkovitost, može preporučiti kao alternativna metoda
