Arbuscular-mycorrhizal symbiosis confers numerous benefits to host plants including improved tolerance to abiotic and biotic stresses. Although the majority of grasses form an arbuscular mycorrhizal symbiosis, little is known of the mycorrhization of Buffel grass (Cenchrus ciliaris). A pot study was conducted in sterilized soil to determine the effect of mycorrhizal inoculation and phosphorus amendment on the biomass production in C. ciliaris. Mycorrhizal fungi used were Gigaspora rosea, Glomus intraradices and Glomus etunicatum. Inoculation with Gigaspora rosea alone, and combined incoculation with Glomus intraradices + Gigaspora rosea and Glomus intraradices + Glomus etunicatum signifi cantly (P<0.05) increased dry biomass in unamended and 25 kg P2O5 ha-1 treatments. Combined inoculation with Glomus intraradices + Gigaspora rosea and Glomus intraradices + Glomus etunicatum showed pronounced (P<0.05) eff ect on dry biomass compared to inoculation with Gigaspora rosea alone in unamended and 25 kg P2O5 ha-1 treatments. Combined inoculation with Glomus intraradices + Glomus etunicatum resulted in signifi cantly (P<0.05) higher dry biomass campared to the combined inoculation with Glomus intraradices + Gigaspora rosea and inoculation with Gigaspora rosea alone in unamended and 25 kg P2O5 ha-1 treatments. Th e results clearly show that inoculation of C. ciliaris plants with mycorrhizal fungi Gigaspora rosea, Glomus intraradices and Glomus etunicatum is highly benefi cial for the growth and biomass production in the absence or presence of P2O5 under sterile soil conditions. Inoculation of C. ciliaris plants with these mycorrizal fungi may help in forage production in marginal and shallow soils of the rangelands of Pakistan