School of Dental Medicine, University of Zagreb, Croatian Dental Society - Croatian Medical Association
Abstract
Svrha rada bila je ispitati genotoksično djelovanje pet dentinskih adheziva: Adper Single Bonda, Adper Single Bonda 2, Prompt L-popa, Excitea i OptiBonda Solo Plus. Ispitivanje genotoksičnosti provedeno je na humanim limfocitima periferne krvi u uvjetima in vitro, ispitane koncentracije adheziva bile su 0,2, 0,5 i 5 μg/ml, a testirana vremena eluacije 1 sat, 24 sata i 5 dana. Genotoksičnost adheziva ispitivala se citogenetičkom metodom - analizom strukturnih aberacija kromosoma, dakle, određivanjem ukupnog broja kromosomskih lomova, kromatidnih lomova i acentričnih fragmenata. Rezultati pokazuju genotoksičnost OptiBonda Solo Plus već u koncentraciji 0,2 μg/ml i to nakon 24-satne eluacije, zatim OptiBonda Solo Plus u koncentraciji 0,5 μg/ml nakon jednosatne eluacije, OptiBonda Solo Plus, Adper Single Bonda 2 i Excitea u koncentraciji 0,5 μg/ml nakon jednodnevne eluacije. U koncentraciji 5 μg/ml nakon jednosatne eluacije genotoksičnost su pokazali OptiBond Solo Plus, Excite, Adper Single Bond 2 i Adper Single Bond, a nakon jednodnevne eluacije svi su ispitivani adhezivi pokazali genotoksičnost. Iz rezultata je jasno da enotoksičnost raste s porastom koncentracije adheziva, a smanjuje se s vremenom. Najveća genotoksičnost zabilježena je nakon 24-satne eluacije.The aim of this study was to examine the genotoxic activity of five dentin bonding agents: Adper Single Bond, Adper Single Bond 2, Prompt L-pop, Excite and Opti-Bond Solo Plus. This in vitro study was performed on human lymphocytes from peripheral blood, and the concentrations of dentin bonding agents tested were 0.2, 0.5 i 5 μg/ml, and elution times tested were 1 hour, 24 hours and 5 days. Genotoxicity testing was done using the Structural chromosomal aberration analysis cytogenic method , which determined the complete number of chromosome breaks, chromatide breaks and acentric fragments. The results showed genotoxicity of Opti-Bond Solo Plus in the 0.2 μg/ml concentration after a 24-hour elution period, then OptiBond Solo Plus in the 0.5 μg/ml concentration after a 1 hour elution period and OptiBond Solo Plus, Adper Single Bond 2 and Excite in the 0.5 μg/ml concentration after a 1 day elution period. In the 5 μg/ml concentration after 1 hour of elution, genotoxic potential was observed in cultures with OptiBond Solo Plus, Excite, Adper Single Bond 2 and Adper Single Bond, while all dentin bonding agents showed genotoxicity at that highest concentration but after 1 day of elution. From the results it is obvious that genotoxicity increases with the concentration of the dentin bonding agent, and decreases over time. The highest genotoxicity was observed after a 24-hour-elution period
