Antibacterial Activity of Calcium Hydroxide Root Canal Sealer (Apexit) - in vitro Study

Abstract

Svrha ovog rada bila je odrediti in vitro antimikrobni učinak Apexita temeljenog na kalcijevu hidroksidu u neposrednom dodiru s bakterijama: Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens i Staphylococcus aureus. Rabljen je Direct contact test (DCT) pri čemu je 10 μl bakterijske suspenzije (106 bakterija) ostavljeno u neposrednom dodiru s punilom 1 sat na 37°C. Pošto je dodan BHI medij (250 μl), mjeren je rast pojedine bakterije nakon 1 sat, 6 sati, 20 sati i 24 sata. Rezultati nakon jednog sata pokazali su da se broj E. coli i P. aeruginosa smanjio na 7-9 x 10 3, S. marcescens na 7 x 10 2 bakterija, a broj S. aureus bio je 1,4 x 10 5. cestosatni uzorci pokazali su da se broj P. aeruginosa smanjio na 10 1, a gram-pozitivne S. aureus na 7,5 x 10 4. Nakon 20 sati preživjela je jedino S. aureus (10 1). Dvadesetčetirisatni uzorci pokazali su potpunu inhibiciju rasta svih ispitivanih vrsta.The aim of this study was to determine the in vitro antimicrobial activity of calcium hydroxide root canal sealer (Apexit) in direct contact with Pseudomonas aeruginosa, Escherichia coli, Serratia marcescens and Staphylococcus aureus. The direct contact test (DCT) was performed. The sealer was mixed and placed on the side wall of microtiter plate wells and 10 μl of bacterial suspension (106 bacteria) was placed onto its surface. Bacteria were in direct contact with the sealer for 1 hour at 37°C. BHI broth (250 μl) was then added and the growth of each strain was measured after 1 hour, 6 hours, 20 hours and 24 hours. After 1 hour the number of E. coli and P. aeruginosa decreased to 7-9 x 10 3 and S. marcescens to 7 x 10 2 bacteria. The number of S. aureus was 1.4 x 10 5 bacteria. Six hour samples showed that the number of P. aeruginosa decreased to 10 1 and gram-positive S. aureus to 7.5 x 10 4. After 20 hours only S. aureus (10 1 bacteria) survived after prior contact with Apexit. The 24-hour samples showed complete bacterial growth inhibition of all the bacterial strains tested in DCT with Apexit