The Mutagenic Potential of Chloroform, Orange Oil, Eucalyptus Oil and Halothane by Salmonella/Microsome Assay

Abstract

Svrha rada bila je ispitati mutagenu aktivnost četiriju komercijalno dostupnih otapala gutaperke Salmonella/mikrosomskim testom. Ispitana su bila otopala: kloroform, narančino ulje, eukaliptusovo ulje i halotan u količinama od 10 μl, 30 μl, 50 μl, 100 μl i 200 μl. Upotrijebljen je standardni Ames test inkorporacije na ploči pri čemu su rabljene bakterije Salmonella typhimurium soja TA 98 i TA 100 s metaboličkom aktivacijom enzimom S9. Rezultati istraživanja pokazuju citotoksičnu aktivnost eukaliptusova ulja u svim koncentracijama, narančina ulja u koncentracijama od 50 μl i većima. Kloroform je pokazao citotoksičnost u koncentracijama od 100 μl i 200 μl. Niti jedno otapalo gutaperke nije pokazala mutagenu aktivnost Ames testom u ispitivanim koncentracijama.The aim of this study was to examine mutagenic activity of four commercially available gutta-percha solvents by means of the Salmonella/ microsome assay. The examined solvents were: chloroform, orange oil, eucalyptus oil and halothane in amounts of 10 μl, 30 μl, 50 μl, 100 μl and 200 μl. Standard plate incorporation Ames test was preformed by using two tester strains of Salmonella typhimurium, TA 98 and TA 100, with metabolic activation of S9. The results showed toxicity of eucalyptus oil in all aliquots, orange oil in aliquots of 50 μl and above and chloroform in aliquots of 100 μl and 200 μl, but all four substances responded negative to the Ames test. These results indicate that the tested solvents do not possess mutagenic activity toward the Salmonella strains used