A total of 9434 fishes, mainly juveniles, belonging to 15 families and 42 species were caught at six stations in the Pantan estuary in the eastern mid Adriatic. Using a 50 m beach seine, 1493 specimens
of 25 species were caught in summer, 2594 specimens of 26 species in autumn, 3626 of 24 species in winter, and 1721 of 30 species in spring. Seven species comprised 87.8% of the total: Atherina boyeri (33.2%), Pomatoschistus marmoratus (28.1%), Liza aurata (8.3%), Liza ramada (7.1%), Aphanius fasciatus (5.5%), Diplodus annularis (2.9%) and Mugil cephalus (2.6%). An ANOSIM2 test for unreplicated 2-way layouts revealed significant differences in species assemblages among the sites (ρav = 0.385, P = 0.007). Stations with similar hydrographic conditions had similar species
richness and assemblages. Statistically significant differences were not noted between seasons (ρav = 0.221, P = 0.057) although some seasonal differences, especially between summer and winter, were observed on nMDS plots. The most important ecological category was planktivorous fishes (39.4%), followed by benthic mesocarnivorous fishes (33.9%), and particulate organic matter
feeders (21.3%). Twenty-two species were permanent estuary residents, 14 opportunists, and six transients. This study demonstrates that the Pantan estuary is an important fish nursery habitat that, although small, has great spatial fish diversity and therefore deserves special protection
