Institute for Medical Research and Occupational Health
Abstract
This recent basic research study used an animal model protocol to assess specific biomarkers of the effect of non-ionising, non-thermal radiation (2450 MHz microwave radiation at 5–15 mW/cm2) on bone marrow, peripheral blood, and bronchoalveolar free cell populations. Of 40 male Wistar rats taken in the study, 20 animals of the experimental group were irradiated for 2 hours a day, 5 days a week, and subsequently killed on days 1, 8, 16, and 30 of the experiment. The remaining 20 rats served as control. All animals were previously intratracheally instilled with biologically inert microspheres to see the influence of irradiation on lung retention kinetics. The cell response to chosen electromagnetic irradiation was followed quantitatively and qualitatively using the standard laboratory methods. The results of peripheral blood cell response suggested a decreasing tendency in total leukocyte count and in relative lymphocyte count in the treated group. A slight increase was also observed in granulocyte count and in the absolute count of peripheral blood erythrocytes over control animals.Ovo pilotsko istraživanje provedeno je na animalnom modelu s namjerom da se uoče specifični biomarkeri učinka neionizirajućeg, netermalnog zračenja (2450 MHz, 5–15 mW/cm2) na stanice koštane srži, periferne krvi i slobodnu staničnu populaciju pluća. U pokus smo uzeli 40 štakora mužjaka soja Wistar, od kojih je kontrolna skupina imala 20 životinja. Pokusnu skupinu životinja zračili smo 2 sata na dan, 5 dana u tjednu. Zatim su životinje žrtvovane 1, 8, 16. i 30. dana pokusa. Svaka je životinja prije početka pokusa intratrahealno instilirana biološki inaktivnim česticama kako bismo dobili uvid u utjecaj zračenja na njihovu retenciju u plućima. Kvantitativni i kvalitativni stanični odgovor na izabrano zračenje pratili smo standardnim laboratorijskim metodama. U usporedbi s kontrolnim životinjama, rezultati staničnog odgovora u perifernoj krvi pokazuju tendenciju snižavanja ukupnog broja leukocita, ali i relativnog broja limfocita u tretiranih životinja te lagano povišenje broja granulocita i apsolutnog broja eritrocita
