Hydrolysis of lactose with -D-galactosidase

Abstract

U ovom radu su ispitivani uvjeti hidrolize laktoze pomoću enzimskog preparata -D-galaktozidaze, a svrha je - primjena sirutke u fermentativnim procesima s kvascem Saccharomyces cerevisiae za kombiniranu proizvodnju alkohola i prehrambenog kvasca. Enzimska hidroliza je provedena na različitim temperaturama, s različitim koncentracijama laktoze u podlozi i različitim količinama dodanog enzimskog preparata. Rezultati pokazuju da je maksimalni stupanj hidrolize postignut u podlozi koja je sadržavala 5-10 % laktoze s dodatkom 2 g/L enzimskog preparata na temperaturi od 40 ºC.The conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactose concentrations in medium and different concentrations of added enzyme. The results show that optimal temperature for hydrolysis was 40°C. The optimal amount of enzyme preparation was 2 gL-1 in lactose medium with 5-10 % lactose