Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

A McMahon; A Nagy,; Allan Bradley; David R Beier; DL Court; E Hughes; F Farley; F Kontgen; GM Hansen; Haydn M Prosser; J Cheng; Jennifer L Moran; K Schuster-Gossler; Kent C Lloyd; L Keskintepe; M Dickie; O Stiedl; P Liu; Q Liang; Qi Liang; R Khisti; R Shimizukawa; S Bultman; Stephen J Pettitt; W Auerbach; William C Skarnes; Xin Y Rairdan

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Authors: A McMahon
A Nagy,
Allan Bradley
David R Beier
DL Court
E Hughes
F Farley
F Kontgen
GM Hansen
Haydn M Prosser
J Cheng
Jennifer L Moran
K Schuster-Gossler
Kent C Lloyd
L Keskintepe
M Dickie
O Stiedl
P Liu
Q Liang
Qi Liang
R Khisti
R Shimizukawa
S Bultman
Stephen J Pettitt
W Auerbach
William C Skarnes
Xin Y Rairdan
Publication date: 1 January 2009
Publisher: Nat Methods
Doi

Abstract

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background