Baoguang Jia

Deyi Yuan

Feng Zou

Lin Zhang

Min Liu

Nan Jiang

Xiaofeng Tan

Yanling Zeng

Zhibo Song

The Scientific World Journal

English

Many flowering plants exhibit an important intraspecific reproductive barrier phenomenon, that is, self-incompatibility (SI), in whichS-RNasegenes play a significant role. To clarify the specific function ofS-RNasegenes in Chinese pears, the full length cDNA ofPbS26-RNasewas isolated by rapid amplification of cDNA ends (RACE) technology from Chinese white pear (Pyrus bretschneideri) cultivar “Hongpisu.” The cDNA sequence forPbS26-RNasewas deposited in GenBank under accession number EU081888. At the amino acid level, thePbS26-RNasedisplayed the highest similarity (96.9%) with PcSa-RNase ofP. communis, and only seven amino acid differences were present in the two S-RNases. Phylogenetic analysis of rosaceous S-RNases indicated that thePbS26-RNaseclustered with maloideous S-RNases, forming a subfamily-specific not a species-specific group. ThePbS26-RNasegene was specifically expressed in the style but not other tissues/organs. The expression level of thePbS26-RNasegene rapidly increased at bell balloon stage (BBS), and then it dropped after pollination. However, the abundance of thePbS26-RNasegene transcript in the style was greater after cross-pollination than after self-pollination. In addition, a method for rapidly detecting thePbS26-RNasegene was developed via allele-specific primers design. The present study could provide a scientific basis for fully clarifying the mechanism of pear SI at the molecular level.</jats:p

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The Scientific World JOURNAL

Sequence Characterization and Spatiotemporal Expression Patterns ofPbS26-RNaseGene in Chinese White Pear (Pyrus bretschneideri)

http://downloads.hindawi.com/journals/tswj/2014/147061.pdf

Sequence Characterization and Spatiotemporal Expression Patterns of
                    PbS

Sequence Characterization and Spatiotemporal Expression Patterns of PbS

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