Chunling Fan

Dan Chen

Hui Wang

JuFang Huang

Kai Huang

Kun Xiong

Lei Shang

Mengqi Zhang

Xiaoxin Yan

BMC Neuroscience

English

AbstractBackgroundChronic lead (Pb) poisoning remains an environmental risk especially for the pediatric population, and it may affect brain development. Immature neurons expressing doublecortin (DCX+) exist around cortical layer II in various mammals, including adult guinea pigs and humans. Using young adult guinea pigs as an experimental model, the present study explored if chronic Pb exposure affects cortical DCX + immature neurons and those around the subventricular and subgranular zones (SVZ, SGZ).ResultsTwo month-old guinea pigs were treated with 0.2% lead acetate in drinking water for 2, 4 and 6 months. Blood Pb levels in these animals reached 10.27 ± 0.62, 16.25 ± 0.78 and 19.03 ± 0.86 μg/dL at the above time points, respectively, relative to ~3 μg/dL in vehicle controls. The density of DCX + neurons was significantly reduced around cortical layer II, SVZ and SGZ in Pb-treated animals surviving 4 and 6 months relative to controls. Bromodeoxyuridine (BrdU) pulse-chasing studies failed to find cellular colocalization of this DNA synthesis indicator in DCX + cells around layer II in Pb-treated and control animals. These cortical immature neurons were not found to coexist with active caspase-3 or Fluoro-Jade C labeling.ConclusionChronic Pb exposure can lead to significant reduction in the number of the immature neurons around cortical layer II and in the conventional neurogenic sites in young adult guinea pigs. No direct evidence could be identified to link the reduced cortical DCX expression with alteration in local neurogenesis or neuronal death.</jats:sec

Chun-Ling Fan

Crossref

Chronic lead exposure reduces doublecortin-expressing immature neurons in young adult guinea pig cerebral cortex

https://bmcneurosci.biomedcentral.com/track/pdf/10.1186/1471-2202-13-82?site=bmcneurosci.biomedcentral.com

Chronic lead exposure reduces doublecortinexpressing immature neurons in young adult guinea pig cerebral cortex

Chronic lead exposure reduces doublecortinexpressing immature neurons in young adult guinea pig cerebral cortex

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