Identification of IGF-1 gene polymorphism using PCR-RFLP for improving goat meat evaluation in Carpatina breed

Abstract

The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve goat meat evaluation. Insulin like growth factor -1 play an important stimulator role in skeletal growth, cell differentiation and metabolism. Also it has an important role in control of hair cycles and it is involved in development of wool fiber. Therefore, it is considered as an appropriate candidate gene for meat quality evaluation. Blood samples were collected from 12 Carpatina kids and DNA was extracted using, Wizard Genomic DNA Purification Kit. Polymorphism of IGF-1 was determined by PCR amplification followed by RFLP method using restriction enzyme Hae III. In the present study homozygous individuals were identified by RFLP with enzyme Hae III which cuts the amplicon in two places obtaining genotype BC with three migration bands at 363, 264 and 99 bp. Restriction enzyme doesn’t cut the amplicon of the homozygous individuals for the genotype BB, and there is no restriction site, so amplified DNA fragment migration can be visualized in one band of 363 bp.  Based on results, two genotypes BB and BC were identified. It was identified homozygous genotype BB with 75% and heterozygous genotype BC with 25%. B allele frequency was 0.87 and for allele C was 0.13. Observed and expected values of IGF-1 genotypes were found in Hardy Weinberg equilibrium, after χ2 test was calculated. Further investigation are necessary to be carried out on a high number of animals in order to discover other mutations correlated with meat quality in Carpatina goat