In the present work synthesis of silver nanoparticles using Alyssum homalocarpum (Fisch. & C.A.Mey.) Boiss. extract has been considered. The methanolic extract of A. homalocarpum was concentrated and analyzed using Gas Chromatogra. Also silver nanoparticles were synthesized by the bio-reduction of silver nitrate solution (1 mm) using the methanol extract. Scanning electron microscopy (SEM), and Fourier transforms infrared spectroscopy (FT-IR), have been used to determine physicochemical properties of silver nanoparticles. α-Glucosidase inhibition assay, α-amylase inhibition activity, and IC50 test have been performed and the results reported. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods have been used to estimate total phenolic and flavonoid content of the extract. Six bacteria and four fungi were used to measure antimicrobial of extract. 9,12,15-Octadecatrien-1-ol, n-Hexadecanoic acid, 2-Pyrazoline, 2,4-Decadienal, and 9,12-Octadecadienoic acid as most important compounds have been determined. The extract showed strong α-glucosidase inhibitory activity (18.01 µg/mL) and also DPPH radical scavenging (IC50: 64 μg/mL). The maximum antibacterial activity was investigated against Salmonella typhi (30.9 mm)
